The rate of progression of radiation-transformed mammary epithelial cells is enhanced after low-dose-rate neutron irradiation

R. L. Ullrich

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Studies in this laboratory have shown enhancement of the mammary tumorigenic effects of neutron irradiation after low-dose-rate neutron exposures. To investigate possible reasons, a mammary cell system was used which allows quantitation of initiated mammary epithelial cells and examination of the progression of these radiation-altered cells toward the neoplastic phenotype. Female BALB/c mice were irradiated with fission-spectrum neutrons at dose rates of 1 rad/minor 1 rad/day. Twenty-four hours or 16 weeks after irradiation, mammary cells were obtained by enzymatic dissociation. Mammary outgrowths were derived by injection of 104 cells into gland-free fat pads of 3-week-old female BALB/c mice. The frequency of ductal dysplasias in outgrowths from cell irradiated at high or low dose rates was similar. Persistence of dysplasias differed markedly. Few of the dysplasias in outhts growths derived from cells irradiated at the high dose rate persisted, while a large fraction of the dysplasias in outgrowths derived from cell irradiated at low dose rate persisted. When cells remained in situ for 16 weeks prior to dissociation a higher frequency of persistent altered cells was also observed in outgrowths derived from cells irradiated at low neutron dose rates. These data suggest that low-dose-rate neutron exposures enhance the probability of progression of carcinogen-altered cells rather than increase the numbers of initiated cells.

Original languageEnglish (US)
Pages (from-to)68-75
Number of pages8
JournalRadiation Research
Volume105
Issue number1
StatePublished - 1986
Externally publishedYes

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Neutrons
neutron irradiation
progressions
breasts
Breast
epithelial cells
irradiation
Epithelial Cells
Radiation
dosage
radiation
cells
neutrons
mice
dissociation
carcinogens
glands
phenotype
neutron spectra
fats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

The rate of progression of radiation-transformed mammary epithelial cells is enhanced after low-dose-rate neutron irradiation. / Ullrich, R. L.

In: Radiation Research, Vol. 105, No. 1, 1986, p. 68-75.

Research output: Contribution to journalArticle

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