DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Clinical Microbiology|
|State||Published - Nov 1 1996|
ASJC Scopus subject areas
- Microbiology (medical)