The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

Xue Jie Yu, Patricia Crocquet-Valdes, Louis C. Cullman, David H. Walker

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

Original languageEnglish (US)
Pages (from-to)2853-2855
Number of pages3
JournalJournal of Clinical Microbiology
Volume34
Issue number11
StatePublished - Nov 1 1996

ASJC Scopus subject areas

  • Microbiology (medical)

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