The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

Xue Jie Yu, Patricia Crocquet-Valdes, Louis C. Cullman, David Walker

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

DNA encoding two repeat units of the 120-kDa protein of Ehrlichia chaffensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

Original languageEnglish (US)
Pages (from-to)2853-2855
Number of pages3
JournalJournal of Clinical Microbiology
Volume34
Issue number11
StatePublished - Nov 1996

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Ehrlichia chaffeensis
Ehrlichia
Indirect Fluorescent Antibody Technique
Recombinant Proteins
Escherichia coli
Sensitivity and Specificity
Antibodies
DNA
Proteins

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool. / Yu, Xue Jie; Crocquet-Valdes, Patricia; Cullman, Louis C.; Walker, David.

In: Journal of Clinical Microbiology, Vol. 34, No. 11, 11.1996, p. 2853-2855.

Research output: Contribution to journalArticle

Yu, Xue Jie ; Crocquet-Valdes, Patricia ; Cullman, Louis C. ; Walker, David. / The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 11. pp. 2853-2855.
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