The rhp6+ gene of Schizosaccharomyces pombe

A structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae

P. Reynolds, M. H M Koken, J. H J Hoeijmakers, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enyzme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxy1-terminal 21 amino acids present in the RAD6 protein are absent in the rAp6+-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

Original languageEnglish (US)
Pages (from-to)1423-1430
Number of pages8
JournalEMBO Journal
Volume9
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

Schizosaccharomyces
Yeast
Saccharomyces cerevisiae
Genes
Yeasts
DNA Repair
Mutagenesis
Repair
DNA
Proteins
Ubiquitin
Eukaryota
DNA Damage
Amino Acids
Defects
Mutation

Keywords

  • DNA repair
  • E2 enzyme
  • RAD6 gens
  • rhp6 gene
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The rhp6+ gene of Schizosaccharomyces pombe : A structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae. / Reynolds, P.; Koken, M. H M; Hoeijmakers, J. H J; Prakash, Satya; Prakash, Louise.

In: EMBO Journal, Vol. 9, No. 5, 1990, p. 1423-1430.

Research output: Contribution to journalArticle

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AU - Koken, M. H M

AU - Hoeijmakers, J. H J

AU - Prakash, Satya

AU - Prakash, Louise

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N2 - The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enyzme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxy1-terminal 21 amino acids present in the RAD6 protein are absent in the rAp6+-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

AB - The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enyzme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxy1-terminal 21 amino acids present in the RAD6 protein are absent in the rAp6+-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

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