The rhp6+ gene of Schizosaccharomyces pombe: A structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae

P. Reynolds, M. H.M. Koken, J. H.J. Hoeijmakers, S. Prakash, L. Prakash

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl-terminal 21 amino acids present in the RAD6 protein are absent in the rhp6+-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.

Original languageEnglish (US)
Pages (from-to)1423-1430
Number of pages8
JournalEMBO Journal
Volume9
Issue number5
DOIs
StatePublished - 1990

Keywords

  • DNA repair
  • E2 enzyme
  • RAD6 gene
  • Schizosaccharomyces pombe
  • rhp6 gene

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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