Abstract
Organisms belonging to the families Rickettsiaceae, Anaplasmataceae, and Coxiellaceae are a diverse group of intracellular bacteria that cause a variety of infections in humans. The serodiagnostic methods of choice for the diagnosis of infection caused by Orientia tsutsugamushi (scrub typhus) include the indirect immunofluorescent and the immunoperoxidase assays. Several serologic methods for the detection of anti-Coxiella antibodies are described. Techniques include microagglutination, complement fixation, microimmunofluorescence, and the enzyme-linked immunosorbent assay (ELISA). Immunohistochemical staining of Rickettsia within formalin fixed paraffin-embedded tissues is useful to demonstrate the presence of the offending agent before reactive serum anti-bodies are present. Immunohistochemical analysis is an effective technique for the visualization of morulae of Ehrlichia species within infected tissues. Cross-reactivity of serum antibodies is also a concern when interpreting serologic data. Finally, establishing a serologic diagnosis is retrospective.
Original language | English (US) |
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Title of host publication | Manual of Molecular and Clinical Laboratory Immunology, 9th Edition |
Subtitle of host publication | Volume 1-2 |
Publisher | wiley |
Pages | 447-457 |
Number of pages | 11 |
Volume | 1 |
ISBN (Electronic) | 9781683674023 |
ISBN (Print) | 9781683673996 |
DOIs | |
State | Published - Jan 1 2024 |
Externally published | Yes |
Keywords
- Anaplasmataceae
- Coxiellaceae
- ELISA
- Immunohistochemical analysis
- Immunoperoxidase assays
- Rickettsiaceae
- Serodiagnostic methods
- Serum antibodies
ASJC Scopus subject areas
- General Medicine
- General Immunology and Microbiology