The role of feature curvature in contact guidance

Anurag Mathur, Simon W. Moore, Michael Sheetz, James Hone

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

This study examines the role of feature curvature in cellular topography sensing. To separate the effects of feature size and curvature we have developed a method to fabricate grooved substrates whose radius of curvature (r) varies from under 10 nm to 400 nm, while all other dimensions are kept constant. With increasing r up to 200 nm mouse embryonic fibroblasts increased their spread area, but reduced their polarization (aspect ratio). Interestingly, on features with r ≈ 200 and 400 nm, which had very little effect on spreading area and polarization, we find that internal structures such as stress fibers are nevertheless still strongly aligned with the topography. These findings are of importance to studies of both tissue engineering and curvature sensing proteins.

Original languageEnglish (US)
Pages (from-to)2595-2601
Number of pages7
JournalActa Biomaterialia
Volume8
Issue number7
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

Keywords

  • Cell morphology
  • Cell spreading
  • Cell-substrate interaction
  • Fibroblasts
  • Surface topography

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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