The role of P-type calcium channels in the depolarization-induced activation of nitric oxide synthase in frontal cortex

Sudarkodi Alagarsamy, Gyorgy Lonart, Kenneth M. Johnson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this study we demonstrate that 50 mM K+ stimulates the conversion of L-[3H]arginine to L-[3H]citrulline and that this effect is blocked by 10 μM Nω-nitro-L-arginine, a nitric oxide synthase inhibitor, and Ca2+-free conditions. Amiloride (1 mM) and low Na+ conditions were used to test the possible involvement of the Na+-Ca2+ exchanger. These treatments were without effect. The calcium channel blockers 10 mM Mg2+, 100 μM Cd2+, and 10 mM Co2+ also blocked the K+ response, suggesting the involvement of voltage-dependent calcium channels (VDCCs). The specific VDCC involved seems to be the P type, as funnel-web spider toxin blocked the response whereas 200 μM Ni2+, 10 μM nifedipine, and 100 nM ω-conotoxin did not.

Original languageEnglish (US)
Pages (from-to)400-403
Number of pages4
JournalJournal of Neurochemistry
Volume62
Issue number1
StatePublished - Jan 1994

Fingerprint

P-Type Calcium Channels
Depolarization
Frontal Lobe
Calcium Channels
Nitric Oxide Synthase
Arginine
Chemical activation
Conotoxins
Citrulline
Amiloride
Calcium Channel Blockers
Nifedipine

Keywords

  • Calcium channel
  • Frontal cortex
  • Funnel-web spider toxin
  • Nitric oxide synthase
  • P-channel
  • Potassium chloride

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

The role of P-type calcium channels in the depolarization-induced activation of nitric oxide synthase in frontal cortex. / Alagarsamy, Sudarkodi; Lonart, Gyorgy; Johnson, Kenneth M.

In: Journal of Neurochemistry, Vol. 62, No. 1, 01.1994, p. 400-403.

Research output: Contribution to journalArticle

Alagarsamy, Sudarkodi ; Lonart, Gyorgy ; Johnson, Kenneth M. / The role of P-type calcium channels in the depolarization-induced activation of nitric oxide synthase in frontal cortex. In: Journal of Neurochemistry. 1994 ; Vol. 62, No. 1. pp. 400-403.
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