The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II

Hongfang Qiu, Eun Park, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The RAD25 gene of Saccharomyces cerevisiae is required for excision repair of ultraviolet-damaged DNA and, in addition, is essential for viability. RAD25 shares a high degree of homology with the human ERCC3/XPBC-encoded protein, and the yeast and human proteins resemble one another in containing the conserved ATPase/DNA helicase sequence motifs. To determine the nature of the essential role of RAD25, we have isolated a recessive temperature-sensitive conditional lethal mutation of the gene and have examined its effect on transcription. Upon shift to the nonpermissive temperature, the rad25 temperature-sensitive (ts) mutant stops growth rapidly and shows a large decrease in the synthesis of poly(A)+ RNA. Transcription of a large number of yeast genes, including HIS3, TRP3, STE2, MET19, RAD23, CDC9, and ACT1 is inhibited at the restrictive temperature in the rad25 ts mutant, and the galactose-inducible synthesis of GAL7 and GAL10 mRNAs is also severely affected by the loss of RAD25 activity. These findings implicate a general requirement of RAD25 in RNA polymerase II transcription.

Original languageEnglish (US)
Pages (from-to)2161-2171
Number of pages11
JournalGenes and Development
Volume7
Issue number11
StatePublished - 1993

Fingerprint

RNA Polymerase II
DNA Repair
Saccharomyces cerevisiae
Temperature
Genes
Lethal Genes
DNA Helicases
Messenger RNA
Fungal Proteins
Galactose
Adenosine Triphosphatases
Yeasts
Mutation
DNA
Growth
Proteins

Keywords

  • DNA repair
  • RAD25 gene
  • RNA polymerase II
  • S. Cerevisiae
  • Transcription
  • UV-damaged DNA

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II. / Qiu, Hongfang; Park, Eun; Prakash, Louise; Prakash, Satya.

In: Genes and Development, Vol. 7, No. 11, 1993, p. 2161-2171.

Research output: Contribution to journalArticle

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N2 - The RAD25 gene of Saccharomyces cerevisiae is required for excision repair of ultraviolet-damaged DNA and, in addition, is essential for viability. RAD25 shares a high degree of homology with the human ERCC3/XPBC-encoded protein, and the yeast and human proteins resemble one another in containing the conserved ATPase/DNA helicase sequence motifs. To determine the nature of the essential role of RAD25, we have isolated a recessive temperature-sensitive conditional lethal mutation of the gene and have examined its effect on transcription. Upon shift to the nonpermissive temperature, the rad25 temperature-sensitive (ts) mutant stops growth rapidly and shows a large decrease in the synthesis of poly(A)+ RNA. Transcription of a large number of yeast genes, including HIS3, TRP3, STE2, MET19, RAD23, CDC9, and ACT1 is inhibited at the restrictive temperature in the rad25 ts mutant, and the galactose-inducible synthesis of GAL7 and GAL10 mRNAs is also severely affected by the loss of RAD25 activity. These findings implicate a general requirement of RAD25 in RNA polymerase II transcription.

AB - The RAD25 gene of Saccharomyces cerevisiae is required for excision repair of ultraviolet-damaged DNA and, in addition, is essential for viability. RAD25 shares a high degree of homology with the human ERCC3/XPBC-encoded protein, and the yeast and human proteins resemble one another in containing the conserved ATPase/DNA helicase sequence motifs. To determine the nature of the essential role of RAD25, we have isolated a recessive temperature-sensitive conditional lethal mutation of the gene and have examined its effect on transcription. Upon shift to the nonpermissive temperature, the rad25 temperature-sensitive (ts) mutant stops growth rapidly and shows a large decrease in the synthesis of poly(A)+ RNA. Transcription of a large number of yeast genes, including HIS3, TRP3, STE2, MET19, RAD23, CDC9, and ACT1 is inhibited at the restrictive temperature in the rad25 ts mutant, and the galactose-inducible synthesis of GAL7 and GAL10 mRNAs is also severely affected by the loss of RAD25 activity. These findings implicate a general requirement of RAD25 in RNA polymerase II transcription.

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