The safety of two Bacillus probiotic strains for human use

Iryna B. Sorokulova, Iryna Pinchuk, Muriel Denayrolles, Irina G. Osipova, Jen M. Huang, Simon M. Cutting, Maria C. Urdaci

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Probiotics based on Bacillus strains have been increasingly proposed for prophylactic and therapeutic use against several gastro-intestinal diseases. We studied safety for two Bacillus strains included in a popular East European probiotic. Bacillus subtilis strain that was sensitive to all antibiotics listed by the European Food Safety Authority. Bacillus licheniformis strain was resistant to chloramphenicol and clindamycin. Both were non-hemolytic and did not produce Hbl or Nhe enterotoxins. No bceT and cytK toxin genes were found. Study of acute toxicity in BALB/c mice demonstrated no treatment-related deaths. The oral LD50 for both strains was more than 2 × 10 11 CFU. Chronic toxicity studies were performed on mice, rabbits, and pigs and showed no signs of toxicity or histological changes in either organs or tissues. We demonstrated that while certain risks may exist for the B. licheniformis strain considering antibiotic resistance, B. subtilis strain may be considered as non-pathogenic and safe for human consumption.

Original languageEnglish (US)
Pages (from-to)954-963
Number of pages10
JournalDigestive Diseases and Sciences
Volume53
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Probiotics
Bacillus subtilis
Bacillus
Safety
Intestinal Diseases
Clindamycin
Food Safety
Enterotoxins
Lethal Dose 50
Therapeutic Uses
Chloramphenicol
Microbial Drug Resistance
Swine
Anti-Bacterial Agents
Rabbits
Genes
Bacillus licheniformis

Keywords

  • Antimicrobials resistance
  • Bacillus
  • Probiotics
  • Safety
  • Toxicity

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Sorokulova, I. B., Pinchuk, I., Denayrolles, M., Osipova, I. G., Huang, J. M., Cutting, S. M., & Urdaci, M. C. (2008). The safety of two Bacillus probiotic strains for human use. Digestive Diseases and Sciences, 53(4), 954-963. https://doi.org/10.1007/s10620-007-9959-1

The safety of two Bacillus probiotic strains for human use. / Sorokulova, Iryna B.; Pinchuk, Iryna; Denayrolles, Muriel; Osipova, Irina G.; Huang, Jen M.; Cutting, Simon M.; Urdaci, Maria C.

In: Digestive Diseases and Sciences, Vol. 53, No. 4, 04.2008, p. 954-963.

Research output: Contribution to journalArticle

Sorokulova, IB, Pinchuk, I, Denayrolles, M, Osipova, IG, Huang, JM, Cutting, SM & Urdaci, MC 2008, 'The safety of two Bacillus probiotic strains for human use', Digestive Diseases and Sciences, vol. 53, no. 4, pp. 954-963. https://doi.org/10.1007/s10620-007-9959-1
Sorokulova IB, Pinchuk I, Denayrolles M, Osipova IG, Huang JM, Cutting SM et al. The safety of two Bacillus probiotic strains for human use. Digestive Diseases and Sciences. 2008 Apr;53(4):954-963. https://doi.org/10.1007/s10620-007-9959-1
Sorokulova, Iryna B. ; Pinchuk, Iryna ; Denayrolles, Muriel ; Osipova, Irina G. ; Huang, Jen M. ; Cutting, Simon M. ; Urdaci, Maria C. / The safety of two Bacillus probiotic strains for human use. In: Digestive Diseases and Sciences. 2008 ; Vol. 53, No. 4. pp. 954-963.
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