The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice

Radnaa Enkhtuya, Tokiharu Sato, Mitsuo Wakasugi, Tuvshintugs Baljinnyam, Hirofumi Miyata, Takeshi Sakurai, Tsukasa Matsunaga, Katsuji Yoshioka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The ultraviolet B (UVB) component of sunlight can cause severe damage to skin cells and even induce skin cancer. Growing evidence indicates that the UVB-induced signaling network is complex and involves diverse cellular processes. In this study, we investigated the role of c-Jun NH2-terminal kinase-associated leucine zipper protein (JLP), a scaffold protein for mitogen-activated protein kinase (MAPK) signaling cascades, in UVB-induced apoptosis. We found that UVB-induced skin epidermal apoptosis was prevented in Jlp knockout (KO) as well as in keratinocyte-specific Jlp KO mice. Analysis of the repair of UVB-induced DNA damage over time showed no evidence for the involvement of JLP in this process. In contrast, UVB-stimulated p38 MAPK activation in the skin was impaired in both Jlp KO and keratinocyte-specific Jlp KO mice. Moreover, topical treatment of UVB-irradiated mouse skin with a p38 inhibitor significantly suppressed the epidermal apoptosis in wild-type mice, but not in Jlp KO mice. Our findings suggest that JLP in skin basal keratinocytes plays an important role in UVB-induced apoptosis by modulating p38 MAPK signaling pathways. This is the first study to show a critical role for JLP in an in vivo response to environmental stimulation.

Original languageEnglish (US)
Pages (from-to)350-358
Number of pages9
JournalGenes to Cells
Volume19
Issue number4
DOIs
StatePublished - 2014
Externally publishedYes

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Apoptosis
Skin
Keratinocytes
Knockout Mice
p38 Mitogen-Activated Protein Kinases
Proteins
Leucine Zippers
JNK Mitogen-Activated Protein Kinases
Sunlight
Skin Neoplasms
Mitogen-Activated Protein Kinases
DNA Damage

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Enkhtuya, R., Sato, T., Wakasugi, M., Baljinnyam, T., Miyata, H., Sakurai, T., ... Yoshioka, K. (2014). The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice. Genes to Cells, 19(4), 350-358. https://doi.org/10.1111/gtc.12135

The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice. / Enkhtuya, Radnaa; Sato, Tokiharu; Wakasugi, Mitsuo; Baljinnyam, Tuvshintugs; Miyata, Hirofumi; Sakurai, Takeshi; Matsunaga, Tsukasa; Yoshioka, Katsuji.

In: Genes to Cells, Vol. 19, No. 4, 2014, p. 350-358.

Research output: Contribution to journalArticle

Enkhtuya, R, Sato, T, Wakasugi, M, Baljinnyam, T, Miyata, H, Sakurai, T, Matsunaga, T & Yoshioka, K 2014, 'The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice', Genes to Cells, vol. 19, no. 4, pp. 350-358. https://doi.org/10.1111/gtc.12135
Enkhtuya R, Sato T, Wakasugi M, Baljinnyam T, Miyata H, Sakurai T et al. The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice. Genes to Cells. 2014;19(4):350-358. https://doi.org/10.1111/gtc.12135
Enkhtuya, Radnaa ; Sato, Tokiharu ; Wakasugi, Mitsuo ; Baljinnyam, Tuvshintugs ; Miyata, Hirofumi ; Sakurai, Takeshi ; Matsunaga, Tsukasa ; Yoshioka, Katsuji. / The scaffold protein JLP plays a key role in regulating ultraviolet B-induced apoptosis in mice. In: Genes to Cells. 2014 ; Vol. 19, No. 4. pp. 350-358.
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