The secreted form of respiratory syncytial virus G glycoprotein helps the virus evade antibody-mediated restriction of replication by acting as an antigen decoy and through effects on Fc receptor-bearing leukocytes

Alexander Bukreyev, Lijuan Yang, Jens Fricke, Lily Cheng, Jerrold M. Ward, Brian R. Murphy, Peter L. Collins

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Respiratory syncytial virus (RSV) readily infects and reinfects during infancy and throughout life, despite maternal antibodies and immunity from prior infection and without the need for significant antigenic change. RSV has two neutralization antigens, the F and G virion glycoproteins. G is expressed in both membrane-bound (mG) and secreted (sG) forms. We investigated whether sG might act as a decoy for neutralizing antibodies by comparing the in vitro neutralization of wild-type (wt) RSV versus recombinant mG RSV expressing only mG. wt RSV indeed was less susceptible than mG RSV to monovalent G-specific and polyvalent RSV-specific antibodies, whereas susceptibility to F-specific antibodies was equivalent. This difference disappeared when the virus preparations were purified to remove sG. Thus, sG appears to function as a neutralization decoy. We evaluated this effect in vivo in mice by comparing the effects of passively transferred antibodies on the pulmonary replication of wt RSV versus mG RSV. Again, wt RSV was less sensitive than mG RSV to G-specific and RSV-specific antibodies; however, a similar difference was also observed with F-specific antibodies. This confirmed that sG helps wt RSV evade the antibodydependent restriction of replication but indicated that in mice, it is not acting primarily as a decoy for G-specific antibodies, perhaps because sG is produced in insufficient quantities in this poorly permissive animal. Rather, we found that the greater sensitivity of mG versus wt RSV to the antiviral effect of passively transferred RSV antibodies required the presence of inflammatory cells in the lung and was Fcγ receptor dependent. Thus, sG helps RSV escape the antibody-dependent restriction of replication via effects as an antigen decoy and as a modulator of leukocytes bearing Fcγ receptors.

Original languageEnglish (US)
Pages (from-to)12191-12204
Number of pages14
JournalJournal of Virology
Volume82
Issue number24
DOIs
StatePublished - Dec 2008
Externally publishedYes

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Respiratory Syncytial Viruses
Fc Receptors
glycoproteins
leukocytes
Leukocytes
Viruses
antigens
Antigens
viruses
receptors
antibodies
Antibodies
neutralization
Respiratory syncytial virus G glycoprotein
maternal immunity
lungs
Lung
mice
Neutralizing Antibodies
infancy

ASJC Scopus subject areas

  • Immunology
  • Virology

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The secreted form of respiratory syncytial virus G glycoprotein helps the virus evade antibody-mediated restriction of replication by acting as an antigen decoy and through effects on Fc receptor-bearing leukocytes. / Bukreyev, Alexander; Yang, Lijuan; Fricke, Jens; Cheng, Lily; Ward, Jerrold M.; Murphy, Brian R.; Collins, Peter L.

In: Journal of Virology, Vol. 82, No. 24, 12.2008, p. 12191-12204.

Research output: Contribution to journalArticle

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