The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids

Yang Qiu, Zhaowei Wang, Yongxiang Liu, Yajuan Han, Meng Miao, Nan Qi, Jie Yang, Hongjie Xia, Xiaofeng Li, Cheng Feng Qin, Yuanyang Hu, Xi Zhou

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3 Scopus citations

Abstract

RNA replication of positive-strand (+)RNA viruses requires the protein-protein interactions among viral replicases and the association of viral replicases with intracellular membranes. Protein A from Wuhan nodavirus (WhNV), which closely associate with mitochondrial membranes, is the sole replicase required for viral RNA replication. Here, we studied the direct effects of mitochondrial membrane lipids (MMLs) on WhNV protein A activity in vitro. Our investigations revealed the self-interaction of WhNV protein A is accomplished via two different patterns (i.e., homotypic and heterotypic self-interactions via different interfaces). MMLs stimulated the protein A self-interaction, and this stimulation exhibited selectivity for specific phospholipids. Moreover, we found that specific phospholipids differently favor the two self-interaction patterns. Furthermore, manipulating specific phospholipid metabolism affected protein A self-interaction and the activity of protein A to replicate RNA in cells. Taken together, our findings reveal the direct effects of membrane lipids on a nodaviral RNA replicase.

Original languageEnglish (US)
Article numbere89628
JournalPloS one
Volume9
Issue number2
DOIs
StatePublished - Feb 25 2014
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Qiu, Y., Wang, Z., Liu, Y., Han, Y., Miao, M., Qi, N., Yang, J., Xia, H., Li, X., Qin, C. F., Hu, Y., & Zhou, X. (2014). The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids. PloS one, 9(2), [e89628]. https://doi.org/10.1371/journal.pone.0089628