The short-term effects of antioxidant and zinc supplements on oxidative stress biomarker levels in plasma: A pilot investigation

Milam A. Brantley, Melissa P. Osborn, Barton J. Sanders, Kasra A. Rezaei, Pengcheng Lu, Chun Li, Ginger L. Milne, Jiyang Cai, Paul Sternberg

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To determine if short-term Age-Related Eye Disease Study (AREDS) antioxidant and zinc supplementation affects biomarkers of oxidative stress, possibly serving as a predictor of their efficacy. Design: Prospective interventional case series. Methods: Nineteen subjects, 12 with intermediate or advanced age-related macular degeneration (AMD) (AREDS categories 3 or 4) and 7 non-AMD controls, were admitted to the Vanderbilt General Clinical Research Center and placed on a controlled diet for 7 days. Antioxidant and zinc supplements were stopped 2 weeks prior to study enrollment. Dietary supplementation with 500 mg vitamin C, 400 IU vitamin E, 15 mg β-carotene, 80 mg zinc oxide, and 2 mg cupric oxide per day was instituted on study day 2. Blood was drawn on study days 2 and 7, and plasma concentrations of cysteine (Cys), cystine (CySS), glutathione (GSH), isoprostane (IsoP), and isofuran (IsoF) were determined. Results: Short-term AREDS supplementation significantly lowered mean plasma levels of CySS in participants on a regulated diet (P =.034). No significant differences were observed for Cys, GSH, IsoP, or IsoF. There were no significant differences between AMD patients and controls. Conclusions: This pilot interventional study shows that a 5-day course of antioxidant and zinc supplements can modify plasma levels of CySS, suggesting that this oxidative stress biomarker could help predict how likely an individual is to benefit from AREDS supplementation. Further, CySS may be useful for the evaluation of new AMD therapies, particularly those hypothesized to affect redox status.

Original languageEnglish (US)
Pages (from-to)1104-1109
Number of pages6
JournalAmerican Journal of Ophthalmology
Volume153
Issue number6
DOIs
StatePublished - Jun 2012
Externally publishedYes

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Cystine
Eye Diseases
Zinc
Macular Degeneration
Oxidative Stress
Antioxidants
Biomarkers
Isoprostanes
Cysteine
Diet
Zinc Oxide
Carotenoids
Dietary Supplements
Vitamin E
Ascorbic Acid
Oxidation-Reduction
Glutathione
Research
Therapeutics

ASJC Scopus subject areas

  • Ophthalmology

Cite this

The short-term effects of antioxidant and zinc supplements on oxidative stress biomarker levels in plasma : A pilot investigation. / Brantley, Milam A.; Osborn, Melissa P.; Sanders, Barton J.; Rezaei, Kasra A.; Lu, Pengcheng; Li, Chun; Milne, Ginger L.; Cai, Jiyang; Sternberg, Paul.

In: American Journal of Ophthalmology, Vol. 153, No. 6, 06.2012, p. 1104-1109.

Research output: Contribution to journalArticle

Brantley, MA, Osborn, MP, Sanders, BJ, Rezaei, KA, Lu, P, Li, C, Milne, GL, Cai, J & Sternberg, P 2012, 'The short-term effects of antioxidant and zinc supplements on oxidative stress biomarker levels in plasma: A pilot investigation', American Journal of Ophthalmology, vol. 153, no. 6, pp. 1104-1109. https://doi.org/10.1016/j.ajo.2011.12.010
Brantley, Milam A. ; Osborn, Melissa P. ; Sanders, Barton J. ; Rezaei, Kasra A. ; Lu, Pengcheng ; Li, Chun ; Milne, Ginger L. ; Cai, Jiyang ; Sternberg, Paul. / The short-term effects of antioxidant and zinc supplements on oxidative stress biomarker levels in plasma : A pilot investigation. In: American Journal of Ophthalmology. 2012 ; Vol. 153, No. 6. pp. 1104-1109.
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