The site of cutaneous infection influences the immunological response and clinical outcome of hamsters infected with Leishmania panamensis

Yaneth Osorio, Peter C. Melby, Claude Pirmez, Bysani Chandrasekar, Nora Guarín, Bruno L. Travi

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We determined that the site of inoculation (foot or snout) influences the clinical evolution and immune responses of hamsters infected with Leishmania (Viannia) panamensis. Hamsters infected in the snout showed (i) a more rapid and severe lesion evolution at multiple time points (P < 0.05), (ii) a more extensive inflammatory infiltrate and tissue necrosis, (iii) a higher tissue parasite burden, (iv) a higher antibody titre (P < 0.01), but lower antigen-specific spleen cell proliferative response (P = 0.02), and (v) a slower response to anti-leishmanial drug treatment (P < 0.002). In both inoculation groups there was co-expression of type 1 (IFN-γ and IL-12) and some type 2 (IL-10 and TGF-β, but not IL-4) cytokines in the cutaneous lesions and spleen. Early in the course of infection, hamsters infected in the snout showed higher expression of splenic IL-10 (P = 0.04) and intralesional IFN-γ (P = 0.02) than foot infections. No expression of IL-12p40 or IL-4 was detected. During the chronic phase, snout lesions expressed more IFN-γ (P = 0.001), IL-12p40 (P = 0.01), IL-10 (P = 0.009) and TGF-β (P = 0.001), and the level of expression of each of these cytokines correlated with lesion size (P ≤ 0.01). These results suggest that the site of infection influences the clinical outcome in experimental cutaneous leishmaniasis, and that the expression of macrophage-deactivating type 2 cytokines and/or an exaggerated type 1 proinflammatory cytokine response may contribute to lesion severity.

Original languageEnglish (US)
Pages (from-to)139-148
Number of pages10
JournalParasite Immunology
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Keywords

  • Cutaneous leishmaniasis
  • Cytokines
  • Immunity
  • Leishmania panamensis

ASJC Scopus subject areas

  • Parasitology
  • Immunology

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