The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms

Jeanne N. Jodoin, Mohammad Shboul, Todd R. Albrecht, Ethan Lee, Eric Wagner, Bruno Reversade, Laura A. Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We previously reported that the small nuclear RNA processing complex, Integrator, is required for dynein recruitment to the nuclear envelope at mitotic onset in cultured human cells. We now report an additional role for INT in ciliogenesis. Depletion of INT subunits from cultured human cells results in loss of primary cilia. We provide evidence that the requirements for INT in dynein localization and ciliogenesis are uncoupled: proteins essential for ciliogenesis are not essential for dynein recruitment to the nuclear envelope, while depletion of known regulators of perinuclear dynein has minimal effects on ciliogenesis. Taken together, our data support a model in which INT ensures proper processing of distinct pools of transcripts encoding components that independently promote perinuclear dynein enrichment and ciliogenesis.

Original languageEnglish (US)
Pages (from-to)1390-1396
Number of pages7
JournalBiology Open
Volume2
Issue number12
DOIs
StatePublished - Dec 15 2013
Externally publishedYes

Fingerprint

small nuclear RNA
Small Nuclear RNA
Dyneins
Nuclear Envelope
nuclear membrane
cilia
Processing
cells
Cultured Cells
Cells
Cilia
proteins
iodonitrotetrazolium
Proteins

Keywords

  • Dynein
  • Primary cilia
  • RNA processing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms. / Jodoin, Jeanne N.; Shboul, Mohammad; Albrecht, Todd R.; Lee, Ethan; Wagner, Eric; Reversade, Bruno; Lee, Laura A.

In: Biology Open, Vol. 2, No. 12, 15.12.2013, p. 1390-1396.

Research output: Contribution to journalArticle

Jodoin, Jeanne N. ; Shboul, Mohammad ; Albrecht, Todd R. ; Lee, Ethan ; Wagner, Eric ; Reversade, Bruno ; Lee, Laura A. / The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms. In: Biology Open. 2013 ; Vol. 2, No. 12. pp. 1390-1396.
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