@article{add63c2281b246508842201b155e3b90,
title = "The Specific α-Neurexin Interactor Calsyntenin-3 Promotes Excitatory and Inhibitory Synapse Development",
abstract = "Perturbations of cell surface synapse-organizing proteins, particularly α-neurexins, contribute to neurodevelopmental and psychiatric disorders. From anunbiased screen, we identify calsyntenin-3 (alcadein-β) as a synapse-organizing protein unique in binding and recruiting α-neurexins, but not β-neurexins. Calsyntenin-3 is present in many pyramidal neurons throughout cortex and hippocampus but is most highly expressed in interneurons. The transmembrane form of calsyntenin-3 can trigger excitatory and inhibitory presynapse differentiation in contacting axons. However, calsyntenin-3-shed ectodomain, which represents about half the calsyntenin-3 pool in brain, suppresses the ability of multiple α-neurexin partners including neuroligin 2 and LRRTM2 to induce presynapse differentiation. Clstn3-/- mice show reductions in excitatory and inhibitory synapse density by confocal and electron microscopy and corresponding deficits in synaptic transmission. These results identify calsyntenin-3 as an α-neurexin-specific binding partner required for normal functional GABAergic and glutamatergic synapse development",
author = "Pettem, \{Katherine L.\} and Daisaku Yokomaku and Lin Luo and Linhoff, \{Michael W.\} and Tuhina Prasad and Connor, \{Steven A.\} and Siddiqui, \{Tabrez J.\} and Hiroshi Kawabe and Fang Chen and Ling Zhang and Gabby Rudenko and Wang, \{Yu Tian\} and Nils Brose and Craig, \{Ann Marie\}",
note = "Funding Information: We thank Xiling Zhou and Nazarine Fernandes for excellent technical assistance, Vivian Lam and Sarah Au-Yeung for contributions for the western blot analysis, Fergil Mills and Dr. Shernaz Bamji for experimental advice and Derrick Horne and Bradford Ross of the Bioimaging Facility for technical assistance with electron microscopy, Michiko Takeda for contributions to mouse colony management, Mika Kishimoto-Suga for experimental advice with antibody generation, and Dr. Robert Holt and team at the Michael Smith Genome Sciences Centre for arraying the cDNA subpool and preparing DNA in 384-well format. This work was supported by the National Institutes of Health (MH070860) and Canada Research Chair salary award to A.M.C., a Natural Sciences and Engineering Research Council of Canada Postgraduate Scholarship to K.L.P., Michael Smith Foundation for Health Research Fellowships to K.L.P. and T.J.S., the German Research Foundation (SPP1365/KA3423/1-1) to H.K, and N.B., the Fritz Thyssen Foundation to H.K., and the National Institutes of Health (MH077303) to G.R. ",
year = "2013",
month = oct,
day = "2",
doi = "10.1016/j.neuron.2013.07.016",
language = "English (US)",
volume = "80",
pages = "113--128",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "1",
}