The splice variant of the V2 vasopressin receptor adopts alternative topologies

Alexis Gonzalez, Mauricio Borquez, Cesar A. Trigo, Marianne Brenet, José M. Sarmiento, Carlos D. Figueroa, Javier Navarro, Carlos B. Gonzalez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The V2 receptor gene encodes two receptor variants by alternative splicing, the canonical V2 receptor (V2a receptor) and V2b. The V2b variant has an amino acid sequence identical to that of the V2a receptor up to the sixth transmembrane domain, but the V2b sequences corresponding to the putative seventh transmembrane domain and the carboxyl terminus are different from those of the V2a receptor. Here we investigate the topology and subcellular distribution of the V2b variant. We found that, in contrast to the V2a receptor, the V2b adopted two topologies: one with six transmembrane segments with the C-terminus on the extracellular side of the membrane and another with seven transmembrane segments with the C-terminus on the intracellular side, similar to typical G-protein-coupled receptors. Furthermore, we observed that both topological isoforms oligomerized with the V2a canonical receptor. Unlike the V2a receptor, V2b did not move to the plasma membrane, but it is retained in the ER-Golgi compartments. These findings indicate that the C-terminal sequence beyond the sixth transmembrane of the V2a is required for the stabilization of the seven-transmembrane topology of the receptor and is also essential for the trafficking of the receptor to the plasma membrane.

Original languageEnglish (US)
Pages (from-to)4981-4986
Number of pages6
JournalBiochemistry
Volume50
Issue number22
DOIs
StatePublished - Jun 7 2011

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Vasopressin Receptors
Cell Membrane
Topology
Cell membranes
Alternative Splicing
G-Protein-Coupled Receptors
Amino Acid Sequence
Protein Isoforms
Membranes
Stabilization
Genes
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gonzalez, A., Borquez, M., Trigo, C. A., Brenet, M., Sarmiento, J. M., Figueroa, C. D., ... Gonzalez, C. B. (2011). The splice variant of the V2 vasopressin receptor adopts alternative topologies. Biochemistry, 50(22), 4981-4986. https://doi.org/10.1021/bi2001278

The splice variant of the V2 vasopressin receptor adopts alternative topologies. / Gonzalez, Alexis; Borquez, Mauricio; Trigo, Cesar A.; Brenet, Marianne; Sarmiento, José M.; Figueroa, Carlos D.; Navarro, Javier; Gonzalez, Carlos B.

In: Biochemistry, Vol. 50, No. 22, 07.06.2011, p. 4981-4986.

Research output: Contribution to journalArticle

Gonzalez, A, Borquez, M, Trigo, CA, Brenet, M, Sarmiento, JM, Figueroa, CD, Navarro, J & Gonzalez, CB 2011, 'The splice variant of the V2 vasopressin receptor adopts alternative topologies', Biochemistry, vol. 50, no. 22, pp. 4981-4986. https://doi.org/10.1021/bi2001278
Gonzalez A, Borquez M, Trigo CA, Brenet M, Sarmiento JM, Figueroa CD et al. The splice variant of the V2 vasopressin receptor adopts alternative topologies. Biochemistry. 2011 Jun 7;50(22):4981-4986. https://doi.org/10.1021/bi2001278
Gonzalez, Alexis ; Borquez, Mauricio ; Trigo, Cesar A. ; Brenet, Marianne ; Sarmiento, José M. ; Figueroa, Carlos D. ; Navarro, Javier ; Gonzalez, Carlos B. / The splice variant of the V2 vasopressin receptor adopts alternative topologies. In: Biochemistry. 2011 ; Vol. 50, No. 22. pp. 4981-4986.
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