The Spliceosome Assembly Pathway in Mammalian Extracts

Sharon F. Jamison, Allen Crow, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A mammalian splicing commitment complex was functionally defined by using a template commitment assay. This complex was partially purified and shown to be a required intermediate for complex A formation. The productive formation of this commitment complex required both splice sites and the polypyrimidine tract. U1 small nuclear ribonucleoprotein (snRNP) was the only spliceosomal U snRNP required for this formation. A protein factor, very likely U2AF, is probably involved in the formation of the splicing commitment complex. From the kinetics of appearance of complex A and complex B, it was previously postulated that complex A represents a functional intermediate in spliceosome assembly. Complex A was partially purified and shown to be a required intermediate for complex B (splicesome) formation. Thus, a spliceosome pathway is for the first time supported by direct biochemical evidence: RNA + U1 snRNP + ?U2 auxiliary factor + ?Y → CC + U2 snRNP + Z → A + U4/6,5 snRNPs + β → B.

Original languageEnglish (US)
Pages (from-to)4279-4287
Number of pages9
JournalMolecular and Cellular Biology
Volume12
Issue number10
StatePublished - Oct 1992
Externally publishedYes

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U1 Small Nuclear Ribonucleoproteins
Spliceosomes
U2 Small Nuclear Ribonucleoproteins
Small Nuclear Ribonucleoproteins
RNA
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The Spliceosome Assembly Pathway in Mammalian Extracts. / Jamison, Sharon F.; Crow, Allen; Garcia-Blanco, Mariano.

In: Molecular and Cellular Biology, Vol. 12, No. 10, 10.1992, p. 4279-4287.

Research output: Contribution to journalArticle

Jamison, Sharon F. ; Crow, Allen ; Garcia-Blanco, Mariano. / The Spliceosome Assembly Pathway in Mammalian Extracts. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 10. pp. 4279-4287.
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