The stalling of transcription at abasic sites is highly mutagenic

Sung Lim Yu, Sung Keun Lee, Robert E. Johnson, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

Abasic (AP) sites represent one of the most frequently formed lesions in DNA. Here, we examine the consequences of the stalling of RNA polymerase II at AP sites in DNA in Saccharomyces cerevisiae. A severe inhibition of transcription occurs in strains that are defective in the removal of AP sites and that also lack the RAD26 gene, a homolog of the human Cockayne syndrome group B (CSB) gene, and, importantly, a dramatic rise in mutagenesis is incurred in such strains. From the various observations presented here, we infer that the stalling of transcription at AP sites is highly mutagenic.

Original languageEnglish (US)
Pages (from-to)382-388
Number of pages7
JournalMolecular and Cellular Biology
Volume23
Issue number1
DOIs
StatePublished - Jan 2003

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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