The structure of barmah forest virus as revealed by cryo-electron microscopy at a 6-angstrom resolution has detailed transmembrane protein architecture and interactions

Victor A. Kostyuchenko, Joanita Jakana, Xiangan Liu, Andrew D. Haddow, Myint Aung, Scott Weaver, Wah Chiu, Shee Mei Lok

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Barmah Forest virus (BFV) is a mosquito-borne alphavirus that infects humans. A 6-Å-resolution cryoelectron microscopy three-dimensional structure of BFV exhibits a typical alphavirus organization, with RNA-containing nucleocapsid surrounded by a bilipid membrane anchored with the surface proteins E1 and E2. The map allows details of the transmembrane regions of E1 and E2 to be seen. The C-terminal end of the E2 transmembrane helix binds to the capsid protein. Following the E2 transmembrane helix, a short α-helical endodomain lies on the inner surface of the lipid envelope. The E2 endodomain interacts with E1 transmembrane helix from a neighboring E1-E2 trimeric spike, thereby acting as a spacer and a linker between spikes. In agreement with previous mutagenesis studies, the endodomain plays an important role in recruiting other E1-E2 spikes to the budding site during virus assembly. The E2 endodomain may thus serve as a target for antiviral drug design.

Original languageEnglish (US)
Pages (from-to)9327-9333
Number of pages7
JournalJournal of Virology
Volume85
Issue number18
DOIs
StatePublished - Sep 2011

Fingerprint

Barmah Forest virus
Alphavirus
Cryoelectron Microscopy
transmembrane proteins
antiviral agents
nucleocapsid
surface proteins
coat proteins
mutagenesis
Culicidae
Proteins
RNA
Virus Assembly
Nucleocapsid
Drug Design
Capsid Proteins
lipids
Mutagenesis
Antiviral Agents
Membrane Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The structure of barmah forest virus as revealed by cryo-electron microscopy at a 6-angstrom resolution has detailed transmembrane protein architecture and interactions. / Kostyuchenko, Victor A.; Jakana, Joanita; Liu, Xiangan; Haddow, Andrew D.; Aung, Myint; Weaver, Scott; Chiu, Wah; Lok, Shee Mei.

In: Journal of Virology, Vol. 85, No. 18, 09.2011, p. 9327-9333.

Research output: Contribution to journalArticle

Kostyuchenko, Victor A. ; Jakana, Joanita ; Liu, Xiangan ; Haddow, Andrew D. ; Aung, Myint ; Weaver, Scott ; Chiu, Wah ; Lok, Shee Mei. / The structure of barmah forest virus as revealed by cryo-electron microscopy at a 6-angstrom resolution has detailed transmembrane protein architecture and interactions. In: Journal of Virology. 2011 ; Vol. 85, No. 18. pp. 9327-9333.
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