The Structure of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase and Its Amino-Terminal Domain

Kyung Choi, Andreas Gallei, Paul Becher, Michael G. Rossmann

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Viral RNA-dependent RNA polymerases (RdRp) differ from DNA-dependent RNA polymerases, DNA-dependent DNA polymerases, and reverse transcriptases in that RdRps contain "fingertips" consisting of several polypeptide strands in the fingers domain interacting with the thumb domain. The crystal structure of bovine viral diarrhea virus (BVDV) RdRp containing an Asn438 duplication shows that the "N-terminal domain," which occurs only in pestiviruses such as BVDV, interacts with the polymerase component of the same polypeptide chain. This contrasts with the domain swapping observed in the previously determined structure of the BVDV NADL strain RdRp. By comparison with the NADL structure and through the use of biochemical data, it is possible that the N-terminal domain, in conjunction with the fingertips, is required to bind and assist the translocation of the RNA template. The partial disorder of the loop containing the additional Asn438 residue may explain the low replication rate of the recombinant compared with the wild-type virus.

Original languageEnglish (US)
Pages (from-to)1107-1113
Number of pages7
JournalStructure
Volume14
Issue number7
DOIs
StatePublished - Jul 2006
Externally publishedYes

Fingerprint

Bovine Viral Diarrhea Viruses
RNA Replicase
Pestivirus
Peptides
RNA-Directed DNA Polymerase
Thumb
Viral RNA
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Fingers
RNA
Viruses

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

The Structure of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase and Its Amino-Terminal Domain. / Choi, Kyung; Gallei, Andreas; Becher, Paul; Rossmann, Michael G.

In: Structure, Vol. 14, No. 7, 07.2006, p. 1107-1113.

Research output: Contribution to journalArticle

Choi, Kyung ; Gallei, Andreas ; Becher, Paul ; Rossmann, Michael G. / The Structure of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase and Its Amino-Terminal Domain. In: Structure. 2006 ; Vol. 14, No. 7. pp. 1107-1113.
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