The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus

Thomas Smith, E. Chase, T. Schmidt, K. L. Perry

Research output: Contribution to journalArticle

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Abstract

The structure of cucumber mosaic virus (CMV; strain Fny) has been determined to a 3.2-Å resolution using X-ray crystallography. Despite the fact that CMV has only 19% capsid protein sequence identity (34% similarity) to cowpea chlorotic mottle virus (CCMV), the core structures of these two members of the Bromoviridae family are highly homologous. As suggested by a previous low-resolution structural study, the 305-Å diameter (maximum) of CMV is ~12 Å larger than that of CCMV. In CCMV, the structures of the A, B, and C subunits are nearly identical except in their N termini. In contrast, the structures of two loops in subunit A of CMV differ from those in B and C. These loops are 6 and 7 residues longer than the analogous regions in CCMV. Unlike that of CCMV, the capsid of CMV does not undergo swelling at pH 7.0 and is stable at pH 9.0. This may be partly due to the fact that the N termini of the B and C subunits form a unique bundle of six amphipathic helices oriented down into the virion core at the threefold axes. In addition, while CCMV has a cluster of aspartic acid residues at the quasi-threefold axis that are proposed to bind metal in a pH-dependent manner this cluster is replaced by complementing acids and bases in CMV. Finally, this structure clearly demonstrates that the residues important for aphid transmission lie at the outermost portion of the βH-βI loop and yields details of the portions of the virus that are hypothesized to mediate binding to aphid mouthparts.

Original languageEnglish (US)
Pages (from-to)7578-7586
Number of pages9
JournalJournal of Virology
Volume74
Issue number16
DOIs
StatePublished - 2000
Externally publishedYes

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Cowpea chlorotic mottle virus
Bromovirus
Cucumovirus
Cucumber mosaic virus
Aphids
Bromoviridae
Aphidoidea
viral morphology
Viral Structures
capsid
Capsid
mouthparts
X Ray Crystallography
Capsid Proteins
aspartic acid
coat proteins
virion
Aspartic Acid
X-ray diffraction
Virion

ASJC Scopus subject areas

  • Immunology

Cite this

The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus. / Smith, Thomas; Chase, E.; Schmidt, T.; Perry, K. L.

In: Journal of Virology, Vol. 74, No. 16, 2000, p. 7578-7586.

Research output: Contribution to journalArticle

Smith, Thomas ; Chase, E. ; Schmidt, T. ; Perry, K. L. / The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus. In: Journal of Virology. 2000 ; Vol. 74, No. 16. pp. 7578-7586.
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