The Structures of Bacteriophages K1E and K1-5 Explain Processive Degradation of Polysaccharide Capsules and Evolution of New Host Specificities

Petr Leiman, Anthony J. Battisti, Valorie D. Bowman, Katharina Stummeyer, Martina Mühlenhoff, Rita Gerardy-Schahn, Dean Scholl, Ian J. Molineux

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

External polysaccharides of many pathogenic bacteria form capsules protecting the bacteria from the animal immune system and phage infection. However, some bacteriophages can digest these capsules using glycosidases displayed on the phage particle. We have utilized cryo-electron microscopy to determine the structures of phages K1E and K1-5 and thereby establish the mechanism by which these phages attain and switch their host specificity. Using a specific glycosidase, both phages penetrate the capsule and infect the neuroinvasive human pathogen Escherichia coli K1. In addition to the K1-specific glycosidase, each K1-5 particle carries a second enzyme that allows it to infect E. coli K5, whose capsule is chemically different from that of K1. The enzymes are organized into a multiprotein complex attached via an adapter protein to the virus portal vertex, through which the DNA is ejected during infection. The structure of the complex suggests a mechanism for the apparent processivity of degradation that occurs as the phage drills through the polysaccharide capsule. The enzymes recognize the adapter protein by a conserved N-terminal sequence, providing a mechanism for phages to acquire different enzymes and thus to evolve new host specificities.

Original languageEnglish (US)
Pages (from-to)836-849
Number of pages14
JournalJournal of Molecular Biology
Volume371
Issue number3
DOIs
StatePublished - Aug 17 2007
Externally publishedYes

Fingerprint

Host Specificity
Bacteriophages
Capsules
Polysaccharides
Glycoside Hydrolases
Enzymes
Escherichia coli
Bacteria
Cryoelectron Microscopy
Multiprotein Complexes
Mandrillus
Infection
Immune System
Proteins
Viruses
DNA

Keywords

  • bacteriophage infection mechanism
  • encapsulated bacteria
  • evolution of viral receptors
  • nanomachine
  • polysialic acid

ASJC Scopus subject areas

  • Virology

Cite this

The Structures of Bacteriophages K1E and K1-5 Explain Processive Degradation of Polysaccharide Capsules and Evolution of New Host Specificities. / Leiman, Petr; Battisti, Anthony J.; Bowman, Valorie D.; Stummeyer, Katharina; Mühlenhoff, Martina; Gerardy-Schahn, Rita; Scholl, Dean; Molineux, Ian J.

In: Journal of Molecular Biology, Vol. 371, No. 3, 17.08.2007, p. 836-849.

Research output: Contribution to journalArticle

Leiman, Petr ; Battisti, Anthony J. ; Bowman, Valorie D. ; Stummeyer, Katharina ; Mühlenhoff, Martina ; Gerardy-Schahn, Rita ; Scholl, Dean ; Molineux, Ian J. / The Structures of Bacteriophages K1E and K1-5 Explain Processive Degradation of Polysaccharide Capsules and Evolution of New Host Specificities. In: Journal of Molecular Biology. 2007 ; Vol. 371, No. 3. pp. 836-849.
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