The tail sheath structure of bacteriophage T4: A molecular machine for infecting bacteria

Anastasia A. Aksyuk, Petr Leiman, Lidia P. Kurochkina, Mikhail M. Shneider, Victor A. Kostyuchenko, Vadim V. Mesyanzhinov, Michael G. Rossmann

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The contractile tail of bacteriophage T4 is a molecular machine that facilitates very high viral infection efficiency. Its major component is a tail sheath, which contracts during infection to less than half of its initial length. The sheath consists of 138 copies of the tail sheath protein, gene product (gp) 18, which surrounds the central non-contractile tail tube. The contraction of the sheath drives the tail tube through the outer membrane, creating a channel for the viral genome delivery. A crystal structure of about three quarters of gp18 has been determined and was fitted into cryo-electron microscopy reconstructions of the tail sheath before and after contraction. It was shown that during contraction, gp18 subunits slide over each other with no apparent change in their structure.

Original languageEnglish (US)
Pages (from-to)821-829
Number of pages9
JournalEMBO Journal
Volume28
Issue number7
DOIs
StatePublished - Apr 8 2009
Externally publishedYes

Fingerprint

Cryoelectron Microscopy
Bacteriophage T4
Bacteriophages
Viral Genome
Virus Diseases
Bacteria
Membranes
Infection
Proteins
Electron microscopy
Genes
Crystal structure

Keywords

  • Bacteriophage T4
  • Cryo-electron microscopy
  • Crystallography
  • Tail sheath contraction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Medicine(all)

Cite this

Aksyuk, A. A., Leiman, P., Kurochkina, L. P., Shneider, M. M., Kostyuchenko, V. A., Mesyanzhinov, V. V., & Rossmann, M. G. (2009). The tail sheath structure of bacteriophage T4: A molecular machine for infecting bacteria. EMBO Journal, 28(7), 821-829. https://doi.org/10.1038/emboj.2009.36

The tail sheath structure of bacteriophage T4 : A molecular machine for infecting bacteria. / Aksyuk, Anastasia A.; Leiman, Petr; Kurochkina, Lidia P.; Shneider, Mikhail M.; Kostyuchenko, Victor A.; Mesyanzhinov, Vadim V.; Rossmann, Michael G.

In: EMBO Journal, Vol. 28, No. 7, 08.04.2009, p. 821-829.

Research output: Contribution to journalArticle

Aksyuk, AA, Leiman, P, Kurochkina, LP, Shneider, MM, Kostyuchenko, VA, Mesyanzhinov, VV & Rossmann, MG 2009, 'The tail sheath structure of bacteriophage T4: A molecular machine for infecting bacteria', EMBO Journal, vol. 28, no. 7, pp. 821-829. https://doi.org/10.1038/emboj.2009.36
Aksyuk AA, Leiman P, Kurochkina LP, Shneider MM, Kostyuchenko VA, Mesyanzhinov VV et al. The tail sheath structure of bacteriophage T4: A molecular machine for infecting bacteria. EMBO Journal. 2009 Apr 8;28(7):821-829. https://doi.org/10.1038/emboj.2009.36
Aksyuk, Anastasia A. ; Leiman, Petr ; Kurochkina, Lidia P. ; Shneider, Mikhail M. ; Kostyuchenko, Victor A. ; Mesyanzhinov, Vadim V. ; Rossmann, Michael G. / The tail sheath structure of bacteriophage T4 : A molecular machine for infecting bacteria. In: EMBO Journal. 2009 ; Vol. 28, No. 7. pp. 821-829.
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