The three-dimensional structure of canine parvovirus and its functional implications

Jun Tsao, Michael S. Chapman, Mavis Agbandje, Walter Keller, Kathy Smith, Hao Wu, Ming Luo, Thomas Smith, Michael G. Rossmann, Richard W. Compans, Colin R. Parrish

Research output: Contribution to journalArticle

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Abstract

The three-dimensional atomic structure of a single-stranded DNA virus has been determined. Infectious virions of canine parvovirus contain 60 protein subunits that are predominantly VP-2. The central structural motif of VP-2 has the same topology (an eight-stranded anti-parallel β barrel) as has been found in many other icosahedral viruses but represents only about one-third of the capsid protein. There is a 22 angstrom (Å) long protrusion on the threefold axes, a 15 A deep canyon circulating about each of the five cylindrical structures at the fivefold axes, and a 15 A deep depression at the twofold axes. By analogy with rhinoviruses, the canyon may be the site of receptor attachment. Residues related to the antigenic properties of the virus are found on the threefold protrusions. Some of the amino termini of VP-2 run to the exterior in full but not empty virions, which is consistent with the observation that some VP-2 polypeptides in full particles can be cleaved by trypsin. Eleven nucleotides are seen in each of 60 symmetry-related pockets on the interior surface of the capsid and together account for 13 percent of the genome.

Original languageEnglish (US)
Pages (from-to)1456-1464
Number of pages9
JournalScience
Volume251
Issue number5000
StatePublished - Mar 22 1991
Externally publishedYes

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Canine Parvovirus
Virion
Viruses
Rhinovirus
DNA Viruses
Capsid
Single-Stranded DNA
Protein Subunits
Capsid Proteins
Trypsin
Nucleotides
Genome
Peptides
17-acetoxy-6-methylene-4-pregnene-3,20-dione

ASJC Scopus subject areas

  • General

Cite this

Tsao, J., Chapman, M. S., Agbandje, M., Keller, W., Smith, K., Wu, H., ... Parrish, C. R. (1991). The three-dimensional structure of canine parvovirus and its functional implications. Science, 251(5000), 1456-1464.

The three-dimensional structure of canine parvovirus and its functional implications. / Tsao, Jun; Chapman, Michael S.; Agbandje, Mavis; Keller, Walter; Smith, Kathy; Wu, Hao; Luo, Ming; Smith, Thomas; Rossmann, Michael G.; Compans, Richard W.; Parrish, Colin R.

In: Science, Vol. 251, No. 5000, 22.03.1991, p. 1456-1464.

Research output: Contribution to journalArticle

Tsao, J, Chapman, MS, Agbandje, M, Keller, W, Smith, K, Wu, H, Luo, M, Smith, T, Rossmann, MG, Compans, RW & Parrish, CR 1991, 'The three-dimensional structure of canine parvovirus and its functional implications', Science, vol. 251, no. 5000, pp. 1456-1464.
Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H et al. The three-dimensional structure of canine parvovirus and its functional implications. Science. 1991 Mar 22;251(5000):1456-1464.
Tsao, Jun ; Chapman, Michael S. ; Agbandje, Mavis ; Keller, Walter ; Smith, Kathy ; Wu, Hao ; Luo, Ming ; Smith, Thomas ; Rossmann, Michael G. ; Compans, Richard W. ; Parrish, Colin R. / The three-dimensional structure of canine parvovirus and its functional implications. In: Science. 1991 ; Vol. 251, No. 5000. pp. 1456-1464.
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