The three-dimensional structure of canine parvovirus and its functional implications

Jun Tsao, Michael S. Chapman, Mavis Agbandje, Walter Keller, Kathy Smith, Hao Wu, Ming Luo, Thomas J. Smith, Michael G. Rossmann, Richard W. Compans, Colin R. Parrish

Research output: Contribution to journalArticle

368 Scopus citations

Abstract

The three-dimensional atomic structure of a single-stranded DNA virus has been determined. Infectious virions of canine parvovirus contain 60 protein subunits that are predominantly VP-2. The central structural motif of VP-2 has the same topology (an eight-stranded anti-parallel β barrel) as has been found in many other icosahedral viruses but represents only about one-third of the capsid protein. There is a 22 angstrom (Å) long protrusion on the threefold axes, a 15 Å deep canyon circulating about each of the five cylindrical structures at the fivefold axes, and a 15 Å deep depression at the twofold axes. By analogy with rhinoviruses, the canyon may be the site of receptor attachment. Residues related to the antigenic properties of the virus are found on the threefold protrusions. Some of the amino termini of VP-2 run to the exterior in full but not empty virions, which is consistent with the observation that some VP-2 polypeptides in full particles can be cleaved by trypsin. Eleven nucleotides are seen in each of 60 symmetry-related pockets on the interior surface of the capsid and together account for 13 percent of the genome.

Original languageEnglish (US)
Pages (from-to)1456-1464
Number of pages9
JournalScience
Volume251
Issue number5000
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • General

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    Tsao, J., Chapman, M. S., Agbandje, M., Keller, W., Smith, K., Wu, H., Luo, M., Smith, T. J., Rossmann, M. G., Compans, R. W., & Parrish, C. R. (1991). The three-dimensional structure of canine parvovirus and its functional implications. Science, 251(5000), 1456-1464. https://doi.org/10.1126/science.2006420