The three-dimensional structure of the Limulus acrosomal process: A dynamic actin bundle

Michael Sherman, Joanita Jakana, Shujun Sun, Paul Matsudaira, Wah Chiu, Michael F. Schmid

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Limulus sperm contains a dynamic macromolecular structure that rapidly extends a 50 μm process called the true discharge. The core of this structure is a bundle of ordered filaments composed of a complex of actin, scruin and calmodulin. We determined its structure by electron crystallographic reconstruction. The three-dimensional map reveals an actin-scruin helix that is azimuthally modulated by the influence of the interactions of a filament with its neighbors. There are a variety of density connections with neighboring filaments involving scruin. Scruin commonly contacts one neighbor, but we observe up to three interfilament connections involving both domains of the 28 scruin molecules in the unit cell. Our structure indicates that promiscuous scruin-scruin contacts are the major determinants of bundle stability in the true discharge. It also suggests that rearrangements would be permitted, which can facilitate the transition from the coiled to the true discharge form.

Original languageEnglish (US)
Pages (from-to)139-149
Number of pages11
JournalJournal of Molecular Biology
Volume294
Issue number1
DOIs
StatePublished - Nov 19 1999
Externally publishedYes

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Horseshoe Crabs
Actins
Calmodulin
Spermatozoa
Electrons

Keywords

  • Acrosomal bundles
  • Actin
  • Electron cryomicroscopy
  • Electron crystallography
  • Scruin

ASJC Scopus subject areas

  • Virology

Cite this

The three-dimensional structure of the Limulus acrosomal process : A dynamic actin bundle. / Sherman, Michael; Jakana, Joanita; Sun, Shujun; Matsudaira, Paul; Chiu, Wah; Schmid, Michael F.

In: Journal of Molecular Biology, Vol. 294, No. 1, 19.11.1999, p. 139-149.

Research output: Contribution to journalArticle

Sherman, Michael ; Jakana, Joanita ; Sun, Shujun ; Matsudaira, Paul ; Chiu, Wah ; Schmid, Michael F. / The three-dimensional structure of the Limulus acrosomal process : A dynamic actin bundle. In: Journal of Molecular Biology. 1999 ; Vol. 294, No. 1. pp. 139-149.
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