The time course of malondialdehyde production following impact injury to rat spinal cord as measured by microdialysis and high pressure liquid chromatography

Hao Qian, Danxia Liu

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

This paper reports a highly sensitive, specific, and reproducible method for the analysis of malondialdehyde (MDA) from microdialysates. The microdialysates were reacted with 2-thiobarbituric acid, and the TBA adducts were separated by HPLC and detected using a fluorescence detector. Butylated hydroxytoluene was used as an antioxidant to minimize formation of artifacts. The time course of MDA production following impact injury to the rat spinal cord was obtained using this improved method. MDA concentrations in the extracellular space gradually increased from a basal level of 20 +/- 3.6 nM to 44 +/- 18.1 nM during the first 2 hr, reached a maximum of 95 +/- 19.8 nM at 5 hr, and then decreased to 36 +/- 9.5 nM at 9 hr. The findings support the hypothesis that spinal cord injury leads to increased membrane lipid peroxidation.

Original languageEnglish (US)
Pages (from-to)1231-1236
Number of pages6
JournalNeurochemical Research
Volume22
Issue number10
DOIs
StatePublished - Oct 25 1997

Keywords

  • HPLC analysis
  • Malondialdehyde
  • Membrane lipid peroxidation
  • Microdialysis sampling
  • Spinal cord injury

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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