The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development

Shao-Jun Tang, Pamela A. Hoodless, Zhan Lu, Martin L. Breitman, Roderick R. McInnes, Jeffrey L. Wrana, Manuel Buchwald

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

TGFβ-related factors are critical regulators of vertebrate mesoderm development. However, the signalling cascades required for their function during this developmental process are poorly defined. Tlx-2 is a homeobox gene expressed in the primitive streak of mouse embryos. Exogenous BMP-2 rapidly activates Tlx-2 expression in the epiblast of E6.5 embryos. A Tlx-2 promoter element responds to BMP-2 signals in P19 cells, and this response is mediated by BMP type I receptors and Smad1. These results suggest that Tlx-2 is a downstream target gene for BMP signalling in the primitive streak where BMP-4 and other TGFβ-related factors are expressed. Furthermore, disruption of Tlx-2 function leads to early embryonic lethality. Similar to BMP4 and ALK3 mutants, the mutant embryos display severe defects in primitive streak and mesoderm formation. These experiments thus define a BMP/Tlx-2 signalling pathway that is required during early mammalian gastrulation.

Original languageEnglish (US)
Pages (from-to)1877-1887
Number of pages11
JournalDevelopment
Volume125
Issue number10
StatePublished - 1998
Externally publishedYes

Fingerprint

Primitive Streak
Homeobox Genes
Mesoderm
Embryonic Structures
Type I Bone Morphogenetic Protein Receptors
Germ Layers
Gastrulation
Vertebrates
Genes

Keywords

  • Bone morphogenetic protein (BMP)
  • Gastrulation
  • Homeobox gene
  • Mesoderm
  • Mouse

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Tang, S-J., Hoodless, P. A., Lu, Z., Breitman, M. L., McInnes, R. R., Wrana, J. L., & Buchwald, M. (1998). The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Development, 125(10), 1877-1887.

The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. / Tang, Shao-Jun; Hoodless, Pamela A.; Lu, Zhan; Breitman, Martin L.; McInnes, Roderick R.; Wrana, Jeffrey L.; Buchwald, Manuel.

In: Development, Vol. 125, No. 10, 1998, p. 1877-1887.

Research output: Contribution to journalArticle

Tang, S-J, Hoodless, PA, Lu, Z, Breitman, ML, McInnes, RR, Wrana, JL & Buchwald, M 1998, 'The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development', Development, vol. 125, no. 10, pp. 1877-1887.
Tang S-J, Hoodless PA, Lu Z, Breitman ML, McInnes RR, Wrana JL et al. The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Development. 1998;125(10):1877-1887.
Tang, Shao-Jun ; Hoodless, Pamela A. ; Lu, Zhan ; Breitman, Martin L. ; McInnes, Roderick R. ; Wrana, Jeffrey L. ; Buchwald, Manuel. / The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. In: Development. 1998 ; Vol. 125, No. 10. pp. 1877-1887.
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