The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development

Shao Jun Tang, Pamela A. Hoodless, Zhan Lu, Martin L. Breitman, Roderick R. McInnes, Jeffrey L. Wrana, Manuel Buchwald

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

TGFβ-related factors are critical regulators of vertebrate mesoderm development. However, the signalling cascades required for their function during this developmental process are poorly defined. Tlx-2 is a homeobox gene expressed in the primitive streak of mouse embryos. Exogenous BMP-2 rapidly activates Tlx-2 expression in the epiblast of E6.5 embryos. A Tlx-2 promoter element responds to BMP-2 signals in P19 cells, and this response is mediated by BMP type I receptors and Smad1. These results suggest that Tlx-2 is a downstream target gene for BMP signalling in the primitive streak where BMP-4 and other TGFβ-related factors are expressed. Furthermore, disruption of Tlx-2 function leads to early embryonic lethality. Similar to BMP4 and ALK3 mutants, the mutant embryos display severe defects in primitive streak and mesoderm formation. These experiments thus define a BMP/Tlx-2 signalling pathway that is required during early mammalian gastrulation.

Original languageEnglish (US)
Pages (from-to)1877-1887
Number of pages11
JournalDevelopment
Volume125
Issue number10
StatePublished - Jun 26 1998
Externally publishedYes

Keywords

  • Bone morphogenetic protein (BMP)
  • Gastrulation
  • Homeobox gene
  • Mesoderm
  • Mouse

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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    Tang, S. J., Hoodless, P. A., Lu, Z., Breitman, M. L., McInnes, R. R., Wrana, J. L., & Buchwald, M. (1998). The Tlx-2 homeobox gene is a downstream target of BMP signalling and is required for mouse mesoderm development. Development, 125(10), 1877-1887.