The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model

Nigel Bourne, David I. Bernstein, James Ireland, Andrew J. Sonderfan, Albert T. Profy, Lawrence R. Stanberry

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Vaginal gel formulations containing the naphthalene sulfonate polymer PRO 2000 are being developed as topical microbicides to protect against infection with sexually transmitted disease (STD) pathogens. A mouse model was used to determine whether PRO 2000 could protect against genital herpes in vivo. Animals received a single intravaginal application of 15 μL of a 10% PRO 2000 aqueous solution or a 4.0% or 0.5% PRO 2000 vaginal gel formulation 20 s prior to intravaginal challenge with 4.0 log10 pfu of herpes simplex virus type 2. Treatment with the 4.0% gel provided complete protection against infection; treatment with the 0.5% gel or 10% solution provided 81% and 80% protection, respectively. Furthermore, the 4% gel provided significant protection even when viral challenge was delayed until 60 min after treatment. This is the first report to show that PRO 2000 can protect against infection with an STD pathogen in vivo.

Original languageEnglish (US)
Pages (from-to)203-205
Number of pages3
JournalJournal of Infectious Diseases
Volume180
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

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Herpes Genitalis
Local Anti-Infective Agents
Foams and Jellies Vaginal Creams
Infection
Gels
Sexually Transmitted Diseases
Human Herpesvirus 2
Polymers
PRO 2000

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Bourne, N., Bernstein, D. I., Ireland, J., Sonderfan, A. J., Profy, A. T., & Stanberry, L. R. (1999). The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model. Journal of Infectious Diseases, 180(1), 203-205. https://doi.org/10.1086/314853

The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model. / Bourne, Nigel; Bernstein, David I.; Ireland, James; Sonderfan, Andrew J.; Profy, Albert T.; Stanberry, Lawrence R.

In: Journal of Infectious Diseases, Vol. 180, No. 1, 1999, p. 203-205.

Research output: Contribution to journalArticle

Bourne, N, Bernstein, DI, Ireland, J, Sonderfan, AJ, Profy, AT & Stanberry, LR 1999, 'The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model', Journal of Infectious Diseases, vol. 180, no. 1, pp. 203-205. https://doi.org/10.1086/314853
Bourne, Nigel ; Bernstein, David I. ; Ireland, James ; Sonderfan, Andrew J. ; Profy, Albert T. ; Stanberry, Lawrence R. / The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model. In: Journal of Infectious Diseases. 1999 ; Vol. 180, No. 1. pp. 203-205.
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