The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma

Brittany C. Parker, Matti J. Annala, David E. Cogdell, Kirsi J. Granberg, Yan Sun, Ping Ji, Xia Li, Joy Gumin, Hong Zheng, Limei Hu, Olli Yli-Harja, Hannu Haapasalo, Tapio Visakorpi, Xiuping Liu, Chang Gong Liu, Raymond Sawaya, Gregory N. Fuller, Kexin Chen, Frederick F. Lang, Matti NykterWei Zhang

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Fusion genes are chromosomal aberrations that are found in many cancers and can be used as prognostic markers and drug targets in clinical practice. Fusions can lead to production of oncogenic fusion proteins or to enhanced expression of oncogenes. Several recent studies have reported that some fusion genes can escape microRNA regulation via 3'-untranslated region (3'-UTR) deletion. We performed whole transcriptome sequencing to identify fusion genes in glioma and discovered FGFR3-TACC3 fusions in 4 of 48 glioblastoma samples from patients both of mixed European and of Asian descent, but not in any of 43 low-grade glioma samples tested. The fusion, caused by tandem duplication on 4p16.3, led to the loss of the 3'-UTR of FGFR3, blocking gene regulation of miR-99a and enhancing expression of the fusion gene. The fusion gene was mutually exclusive with EGFR, PDGFR, or MET amplification. Using cultured glioblastoma cells and a mouse xenograft model, we found that fusion protein expression promoted cell proliferation and tumor progression, while WT FGFR3 protein was not tumorigenic, even under forced overexpression. These results demonstrated that the FGFR3-TACC3 gene fusion is expressed in human cancer and generates an oncogenic protein that promotes tumorigenesis in glioblastoma.

Original languageEnglish (US)
Pages (from-to)855-865
Number of pages11
JournalJournal of Clinical Investigation
Volume123
Issue number2
DOIs
StatePublished - Feb 1 2013
Externally publishedYes

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Gene Fusion
Glioblastoma
3' Untranslated Regions
Glioma
Receptor, Fibroblast Growth Factor, Type 3
Neoplasms
Proteins
MicroRNAs
Oncogenes
Transcriptome
Heterografts
Chromosome Aberrations
Cultured Cells
Carcinogenesis
Cell Proliferation
Gene Expression
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Parker, B. C., Annala, M. J., Cogdell, D. E., Granberg, K. J., Sun, Y., Ji, P., ... Zhang, W. (2013). The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma. Journal of Clinical Investigation, 123(2), 855-865. https://doi.org/10.1172/JCI67144

The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma. / Parker, Brittany C.; Annala, Matti J.; Cogdell, David E.; Granberg, Kirsi J.; Sun, Yan; Ji, Ping; Li, Xia; Gumin, Joy; Zheng, Hong; Hu, Limei; Yli-Harja, Olli; Haapasalo, Hannu; Visakorpi, Tapio; Liu, Xiuping; Liu, Chang Gong; Sawaya, Raymond; Fuller, Gregory N.; Chen, Kexin; Lang, Frederick F.; Nykter, Matti; Zhang, Wei.

In: Journal of Clinical Investigation, Vol. 123, No. 2, 01.02.2013, p. 855-865.

Research output: Contribution to journalArticle

Parker, BC, Annala, MJ, Cogdell, DE, Granberg, KJ, Sun, Y, Ji, P, Li, X, Gumin, J, Zheng, H, Hu, L, Yli-Harja, O, Haapasalo, H, Visakorpi, T, Liu, X, Liu, CG, Sawaya, R, Fuller, GN, Chen, K, Lang, FF, Nykter, M & Zhang, W 2013, 'The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma', Journal of Clinical Investigation, vol. 123, no. 2, pp. 855-865. https://doi.org/10.1172/JCI67144
Parker, Brittany C. ; Annala, Matti J. ; Cogdell, David E. ; Granberg, Kirsi J. ; Sun, Yan ; Ji, Ping ; Li, Xia ; Gumin, Joy ; Zheng, Hong ; Hu, Limei ; Yli-Harja, Olli ; Haapasalo, Hannu ; Visakorpi, Tapio ; Liu, Xiuping ; Liu, Chang Gong ; Sawaya, Raymond ; Fuller, Gregory N. ; Chen, Kexin ; Lang, Frederick F. ; Nykter, Matti ; Zhang, Wei. / The tumorigenic FGFR3-TACC3 gene fusion escapes miR-99a regulation in glioblastoma. In: Journal of Clinical Investigation. 2013 ; Vol. 123, No. 2. pp. 855-865.
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