The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis

A. R. Talley, F. Garcia-Ferrer, K. A. Laycock, M. Loeffelholz, J. S. Pepose

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Ocular swabs from 30 consecutive patients with follicular conjunctivitis were screened for Chlamydia trachomatis, herpes simplex virus, and adenovirus by a variety of laboratory techniques. For the detection of C. trachomatis, we compared two polymerase chain reaction methods, McCoy cell culture isolation, and the direct fluorescein-conjugated monoclonal antibody test. Four of 30 patients tested positive for C. trachomatis by using both conventional and biotinylated polymerase chain reaction methods. Two of the four patients were also McCoy cell culture-positive for C. trachomatis and one of four patients tested positive by using a fluorescein-conjugated chlamydial monoclonal antibody test. All four patients responded to oral antibiotic treatment. On follow-up testing, all four patients were polymerase chain reaction-negative, McCoy cell culture-negative, and fluorescein- conjugated antibody test-negative for C. trachomatis. The polymerase chain reaction appears to be an equally specific and more sensitive method than McCoy cell culture or fluorescein-conjugated antibody testing for the detection of C. trachomatis from ocular specimens.

Original languageEnglish (US)
Pages (from-to)685-692
Number of pages8
JournalAmerican Journal of Ophthalmology
Volume114
Issue number6
StatePublished - 1992
Externally publishedYes

Fingerprint

Keratoconjunctivitis
Chlamydia trachomatis
Fluorescein
Polymerase Chain Reaction
Cell Culture Techniques
Monoclonal Antibodies
Conjunctivitis
Antibodies
Cell Separation
Simplexvirus
Adenoviridae
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Talley, A. R., Garcia-Ferrer, F., Laycock, K. A., Loeffelholz, M., & Pepose, J. S. (1992). The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. American Journal of Ophthalmology, 114(6), 685-692.

The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. / Talley, A. R.; Garcia-Ferrer, F.; Laycock, K. A.; Loeffelholz, M.; Pepose, J. S.

In: American Journal of Ophthalmology, Vol. 114, No. 6, 1992, p. 685-692.

Research output: Contribution to journalArticle

Talley, AR, Garcia-Ferrer, F, Laycock, KA, Loeffelholz, M & Pepose, JS 1992, 'The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis', American Journal of Ophthalmology, vol. 114, no. 6, pp. 685-692.
Talley AR, Garcia-Ferrer F, Laycock KA, Loeffelholz M, Pepose JS. The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. American Journal of Ophthalmology. 1992;114(6):685-692.
Talley, A. R. ; Garcia-Ferrer, F. ; Laycock, K. A. ; Loeffelholz, M. ; Pepose, J. S. / The use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. In: American Journal of Ophthalmology. 1992 ; Vol. 114, No. 6. pp. 685-692.
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