The use of potassium ferricyanide in neural fixation

L. A. Langford, R. E. Coggeshall

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The present study suggests a mixture of buffered osmic acid and 1.5% potassium ferricyanide as a post-fixation to improve the fixation of neural tissue. This procedure results in an improved preservation of membranes as well as cytoplasm and cytoplasmic organelles. It is to be emphasized that the quality of the initial perfusion is the primary determinant of quality of the fixation, but the addition of 1.5% potassium ferricyanide to post-fixation fluid makes good fixation better and allows data to be gathered from otherwise unusable material.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalAnatomical Record
Volume197
Issue number3
StatePublished - 1980
Externally publishedYes

Fingerprint

ferricyanides
potassium
Tissue Fixation
Osmium Tetroxide
Organelles
organelles
nerve tissue
Cytoplasm
cytoplasm
Perfusion
Membranes
acids
potassium ferricyanide
fluids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Langford, L. A., & Coggeshall, R. E. (1980). The use of potassium ferricyanide in neural fixation. Anatomical Record, 197(3), 297-303.

The use of potassium ferricyanide in neural fixation. / Langford, L. A.; Coggeshall, R. E.

In: Anatomical Record, Vol. 197, No. 3, 1980, p. 297-303.

Research output: Contribution to journalArticle

Langford, LA & Coggeshall, RE 1980, 'The use of potassium ferricyanide in neural fixation', Anatomical Record, vol. 197, no. 3, pp. 297-303.
Langford LA, Coggeshall RE. The use of potassium ferricyanide in neural fixation. Anatomical Record. 1980;197(3):297-303.
Langford, L. A. ; Coggeshall, R. E. / The use of potassium ferricyanide in neural fixation. In: Anatomical Record. 1980 ; Vol. 197, No. 3. pp. 297-303.
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