The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice

Greta V.S. Jerzak; Kristen Bernard; Laura D. Kramer; Pei Yong Shi; Gregory D. Ebel

doi:10.1016/j.virol.2006.10.029

The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice

Greta V.S. Jerzak, Kristen Bernard, Laura D. Kramer, Pei Yong Shi, Gregory D. Ebel

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

102 Scopus citations

Abstract

To define the impact of mosquitoes and birds on intrahost WNV population dynamics, the mutant spectra that arose as a result of 20 serial in vivo passages in Culex pipiens and young chickens were examined. Genetically homogeneous WNV was serially passaged 20 times in each host. Genetic diversity was greater in mosquito-passaged WNV compared to chicken-passaged WNV. Changes in the viral consensus sequence occurred in WNV passaged in mosquitoes earlier and more frequently than in chicken-passaged WNV. Analysis of synonymous and nonsynonymous variation suggested that purifying selection was relaxed during passage in mosquitoes. Mortality in mice was significantly negatively correlated with the size of the WNV mutant spectrum. These studies suggest that mosquitoes serve as sources for WNV genetic diversity, that birds are selective sieves, and that both the consensus sequence and the mutant spectrum contribute to WNV phenotype.

Original language	English (US)
Pages (from-to)	469-476
Number of pages	8
Journal	Virology
Volume	360
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2006.10.029
State	Published - Apr 10 2007

Keywords

Arbovirus
Culex
Evolution
Flavivirus
Quasispecies
West Nile virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2006.10.029

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title = "The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice",

abstract = "To define the impact of mosquitoes and birds on intrahost WNV population dynamics, the mutant spectra that arose as a result of 20 serial in vivo passages in Culex pipiens and young chickens were examined. Genetically homogeneous WNV was serially passaged 20 times in each host. Genetic diversity was greater in mosquito-passaged WNV compared to chicken-passaged WNV. Changes in the viral consensus sequence occurred in WNV passaged in mosquitoes earlier and more frequently than in chicken-passaged WNV. Analysis of synonymous and nonsynonymous variation suggested that purifying selection was relaxed during passage in mosquitoes. Mortality in mice was significantly negatively correlated with the size of the WNV mutant spectrum. These studies suggest that mosquitoes serve as sources for WNV genetic diversity, that birds are selective sieves, and that both the consensus sequence and the mutant spectrum contribute to WNV phenotype.",

keywords = "Arbovirus, Culex, Evolution, Flavivirus, Quasispecies, West Nile virus",

author = "Jerzak, {Greta V.S.} and Kristen Bernard and Kramer, {Laura D.} and Shi, {Pei Yong} and Ebel, {Gregory D.}",

note = "Funding Information: The authors thank David Young, Corey Bennett, Pamela Chin and Amy Lovelace for technical assistance. This work was supported by NIH. The work was supported in part by funds from the National Institute of Allergy and Infectious Disease, National Institutes of Health, under grant AI055609 and contract N01-AI25490. The BSL-3 animal facility at the Wadsworth Center was used, which is funded in part by the Northeast Biodefense Center's animal core on the NIH/NIAID award U54A17158. Sequencing was performed at the Wadsworth Center Molecular Genetics Core facility, and cell cultures were supplied by the Cell Culture facility at the Wadsworth Center.",

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The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice

Abstract

Keywords

ASJC Scopus subject areas

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