The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA

Aparna Ramachandran, Divya Nandakumar, Aishwarya P. Deshpande, Thomas P. Lucas, Ramanagouda R-Bhojappa, Guo Qing Tang, Kevin Raney, Yuhui Yin, Smita S. Patel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Primases use single-stranded (ss) DNAs as templates to synthesize short oligoribonucleotide primers that initiate lagging strand DNA synthesis or reprime DNA synthesis after replication fork collapse, but the origin of this activity in the mitochondria remains unclear. Herein, we show that the Saccharomyces cerevisiae mitochondrial RNA polymerase (Rpo41) and its transcription factor (Mtf1) is an efficient primase that initiates DNA synthesis on ssDNA coated with the yeast mitochondrial ssDNA-binding protein, Rim1. Both Rpo41 and Rpo41-Mtf1 can synthesize short and long RNAs on ssDNA template and prime DNA synthesis by the yeast mitochondrial DNA polymerase Mip1. However, the ssDNA-binding protein Rim1 severely inhibits the RNA synthesis activity of Rpo41, but not the Rpo41-Mtf1 complex, which continues to prime DNA synthesis efficiently in the presence of Rim1. We show that RNAs as short as 10-12 nt serve as primers for DNA synthesis. Characterization of the RNA-DNA products shows that Rpo41 and Rpo41-Mtf1 have slightly different priming specificity. However, both prefer to initiate with ATP from short priming sequences such as 3′-TCC, TTC, and TTT, and the consensus sequence is 3′-Pu(Py)2-3. Based on our studies, we propose that Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA.

Original languageEnglish (US)
Pages (from-to)16828-16839
Number of pages12
JournalJournal of Biological Chemistry
Volume291
Issue number32
DOIs
StatePublished - Aug 5 2016

Fingerprint

Single-Stranded DNA
DNA-Directed RNA Polymerases
Yeast
Transcription Factors
Yeasts
DNA Primase
DNA
RNA
Carrier Proteins
Oligoribonucleotides
DNA Primers
Consensus Sequence
DNA-Directed DNA Polymerase
Mitochondrial DNA
Mitochondria
Saccharomyces cerevisiae
mitochondrial RNA
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Ramachandran, A., Nandakumar, D., Deshpande, A. P., Lucas, T. P., R-Bhojappa, R., Tang, G. Q., ... Patel, S. S. (2016). The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA. Journal of Biological Chemistry, 291(32), 16828-16839. https://doi.org/10.1074/jbc.M116.740282

The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA. / Ramachandran, Aparna; Nandakumar, Divya; Deshpande, Aishwarya P.; Lucas, Thomas P.; R-Bhojappa, Ramanagouda; Tang, Guo Qing; Raney, Kevin; Yin, Yuhui; Patel, Smita S.

In: Journal of Biological Chemistry, Vol. 291, No. 32, 05.08.2016, p. 16828-16839.

Research output: Contribution to journalArticle

Ramachandran, A, Nandakumar, D, Deshpande, AP, Lucas, TP, R-Bhojappa, R, Tang, GQ, Raney, K, Yin, Y & Patel, SS 2016, 'The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA', Journal of Biological Chemistry, vol. 291, no. 32, pp. 16828-16839. https://doi.org/10.1074/jbc.M116.740282
Ramachandran, Aparna ; Nandakumar, Divya ; Deshpande, Aishwarya P. ; Lucas, Thomas P. ; R-Bhojappa, Ramanagouda ; Tang, Guo Qing ; Raney, Kevin ; Yin, Yuhui ; Patel, Smita S. / The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 32. pp. 16828-16839.
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