Theoretical and Experimental Prospects for Protein Identification Based Solely on Accurate Mass Measurement

Fei He, Mark Emmett, Kristina Håkansson, Christopher L. Hendrickson, Alan G. Marshall

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We discuss the theoretical and experimental potential and limitations of protein identification by mass measurement of proteolytic peptides and database searching. For peptides differing in composition by one (or two or three) amino acids, a surprisingly high number turn out to have isomers: 10% (or 29% or 53%), considering the 20 common amino acids with equal relative abundance. Even if isomers differing by leucine/isoleucine are excluded, the latter numbers are 14% and 38%-those isomeric peptides cannot be distinguished based on mass alone, and tandem mass spectrometry and/or other additional constraints are needed. However, for nominally isobaric peptides, the mass accuracy and resolving power of broadband Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry theoretically and experimentally suffice to resolve virtually all peptide doublets differing by up to two amino acids-including the smallest mass difference of 3.4 mDa. We demonstrate experimental resolution of another pair of peptides differing by 11 mDa, even when present in a complex mixture of hundreds of other peptides.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalJournal of Proteome Research
Volume3
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Peptides
Proteins
Amino Acids
Isomers
Mass spectrometry
Cyclotrons
Cyclotron resonance
Isoleucine
Optical resolving power
Fourier Analysis
Tandem Mass Spectrometry
Complex Mixtures
Leucine
Mass Spectrometry
Fourier transforms
Databases
Ions
Chemical analysis

Keywords

  • Accurate mass, elemental composition
  • Chemical formula
  • Exact mass
  • FT-ICR
  • FTICR
  • FTMS
  • Mass resolution
  • Mass resolving power
  • Mutation
  • Peptide
  • Post-translational modification
  • Protein database
  • Protein identification

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Theoretical and Experimental Prospects for Protein Identification Based Solely on Accurate Mass Measurement. / He, Fei; Emmett, Mark; Håkansson, Kristina; Hendrickson, Christopher L.; Marshall, Alan G.

In: Journal of Proteome Research, Vol. 3, No. 1, 01.2004, p. 61-67.

Research output: Contribution to journalArticle

He, Fei ; Emmett, Mark ; Håkansson, Kristina ; Hendrickson, Christopher L. ; Marshall, Alan G. / Theoretical and Experimental Prospects for Protein Identification Based Solely on Accurate Mass Measurement. In: Journal of Proteome Research. 2004 ; Vol. 3, No. 1. pp. 61-67.
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