TY - JOUR
T1 - Theranostic verteporfin- loaded lipid-polymer liposome for photodynamic applications
AU - de Oliveira, Daphne Christine Salles
AU - de Freitas, Camila Fabiano
AU - Calori, Italo Rodrigo
AU - Goncalves, Renato Sonchini
AU - Cardinali, Camila Aparecida Errerias Fernandes
AU - Malacarne, Luis Carlos
AU - Ravanelli, Maria Ida
AU - de Oliveira, Hueder Paulo Moises
AU - Tedesco, Antonio Claudio
AU - Caetano, Wilker
AU - Hioka, Noboru
AU - Tessaro, André Luiz
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11
Y1 - 2020/11
N2 - In this study we report a novel theranostic lipid-polymer liposome, obtained from DPPC and the triblock copolymer F127 covalently modified with 5(6)-carboxyfluorescein (CF) for photodynamic applications. Due to the presence of F127, small unilamellar vesicle (SUV) liposomes were synthesized by a simple and fast thin-film hydration method without the need for an extrusion process. The vesicles have around 100 nm, low polydispersity and superb solution stability. The clinically used photosensitizer verteporfin (VP) was entrapped into the vesicles, mostly in monomeric form, with 90% loading efficiency. Stern-Volmer and fluorescence lifetime assays showed heterogeneous distribution of the VP and CF into the vesicles, ensuring the integrity of their individual photophysical properties. The theranostic properties were entirely photoactivatable and can be trigged by a unique wavelength (470 nm). The feasibility of the system was tested against the Glioblastoma multiforme cell line T98G. Cellular uptake by time-resolved fluorescence microscopy showed monomerized VP (monoexponential decay, 6.0 ns) at nucleus level, while CF was detected at the membrane by fluorescence microscopy. The strategy's success was supported by the reduction of 98% in the viability of T98G cells by the photoactivated lipid-polymer liposome with [VP] = 1.0 μmol L−1. Therefore, the novel theranostic liposome is a potential system for use in cancer and ocular disease therapies.
AB - In this study we report a novel theranostic lipid-polymer liposome, obtained from DPPC and the triblock copolymer F127 covalently modified with 5(6)-carboxyfluorescein (CF) for photodynamic applications. Due to the presence of F127, small unilamellar vesicle (SUV) liposomes were synthesized by a simple and fast thin-film hydration method without the need for an extrusion process. The vesicles have around 100 nm, low polydispersity and superb solution stability. The clinically used photosensitizer verteporfin (VP) was entrapped into the vesicles, mostly in monomeric form, with 90% loading efficiency. Stern-Volmer and fluorescence lifetime assays showed heterogeneous distribution of the VP and CF into the vesicles, ensuring the integrity of their individual photophysical properties. The theranostic properties were entirely photoactivatable and can be trigged by a unique wavelength (470 nm). The feasibility of the system was tested against the Glioblastoma multiforme cell line T98G. Cellular uptake by time-resolved fluorescence microscopy showed monomerized VP (monoexponential decay, 6.0 ns) at nucleus level, while CF was detected at the membrane by fluorescence microscopy. The strategy's success was supported by the reduction of 98% in the viability of T98G cells by the photoactivated lipid-polymer liposome with [VP] = 1.0 μmol L−1. Therefore, the novel theranostic liposome is a potential system for use in cancer and ocular disease therapies.
KW - Fluorescence
KW - Photodynamic therapy
KW - Theranostic liposome
KW - Triblock copolymer
KW - Verteporfin
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U2 - 10.1016/j.jphotobiol.2020.112039
DO - 10.1016/j.jphotobiol.2020.112039
M3 - Article
C2 - 33002779
AN - SCOPUS:85091631136
SN - 1011-1344
VL - 212
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
M1 - 112039
ER -