Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice

Hiroyuki Kobayashi, Makiko Kobayashi, Tokuichiro Utsunomiya, David Herndon, Richard B. Pollard, Fujio Suzuki

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effect of combination therapy between IL-12 and soluble IL-4R (sIL- 4R) on the established infection of HSV-1 in thermally injured mice (TI mice) was investigated. All of the TI mice infected with lethal amounts of HSV-1 died when IL-12 was given therapeutically at a dose of 500 U/mouse. However, 80% of these mice treated prophylactically with IL-12 survived compared with 0% survival of the same mice treated with saline. The therapeutic administration of IL-12 to TI mice currently infected with HSV-1 caused an 80% survival of these mice when the treatment was combined with sIL-4R. Although IL-12 did not stimulate IFN-γ production in cultures of splenic T cells from TI mice, IFN-γ was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R. After stimulation with anti-CD3 mAb, splenic T cells from TI mice with the established infection of HSV-1 produced IL-4 into their culture fluids. However, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination. The results obtained herein indicate that the efficacies of the combination therapy against the established infection of HSV-1 may result from the IFN- γ production stimulated by IL-12 in TI mice that are treated with sIL-4R for reducing burn-associated type 2 T cell responses.

Original languageEnglish (US)
Pages (from-to)7148-7154
Number of pages7
JournalJournal of Immunology
Volume162
Issue number12
StatePublished - Jun 15 1999

Fingerprint

Interleukin-4 Receptors
Human Herpesvirus 1
Therapeutic Uses
Interleukin-12
Infection
T-Lymphocytes
Interleukin-4
Burns

ASJC Scopus subject areas

  • Immunology

Cite this

Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice. / Kobayashi, Hiroyuki; Kobayashi, Makiko; Utsunomiya, Tokuichiro; Herndon, David; Pollard, Richard B.; Suzuki, Fujio.

In: Journal of Immunology, Vol. 162, No. 12, 15.06.1999, p. 7148-7154.

Research output: Contribution to journalArticle

Kobayashi, Hiroyuki ; Kobayashi, Makiko ; Utsunomiya, Tokuichiro ; Herndon, David ; Pollard, Richard B. ; Suzuki, Fujio. / Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice. In: Journal of Immunology. 1999 ; Vol. 162, No. 12. pp. 7148-7154.
@article{ee58476593bd46a6b7ed08373be692c4,
title = "Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice",
abstract = "The effect of combination therapy between IL-12 and soluble IL-4R (sIL- 4R) on the established infection of HSV-1 in thermally injured mice (TI mice) was investigated. All of the TI mice infected with lethal amounts of HSV-1 died when IL-12 was given therapeutically at a dose of 500 U/mouse. However, 80{\%} of these mice treated prophylactically with IL-12 survived compared with 0{\%} survival of the same mice treated with saline. The therapeutic administration of IL-12 to TI mice currently infected with HSV-1 caused an 80{\%} survival of these mice when the treatment was combined with sIL-4R. Although IL-12 did not stimulate IFN-γ production in cultures of splenic T cells from TI mice, IFN-γ was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R. After stimulation with anti-CD3 mAb, splenic T cells from TI mice with the established infection of HSV-1 produced IL-4 into their culture fluids. However, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination. The results obtained herein indicate that the efficacies of the combination therapy against the established infection of HSV-1 may result from the IFN- γ production stimulated by IL-12 in TI mice that are treated with sIL-4R for reducing burn-associated type 2 T cell responses.",
author = "Hiroyuki Kobayashi and Makiko Kobayashi and Tokuichiro Utsunomiya and David Herndon and Pollard, {Richard B.} and Fujio Suzuki",
year = "1999",
month = "6",
day = "15",
language = "English (US)",
volume = "162",
pages = "7148--7154",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - Therapeutic protective effects of IL-12 combined with soluble IL-4 receptor against established infections of herpes simplex virus type 1 in thermally injured mice

AU - Kobayashi, Hiroyuki

AU - Kobayashi, Makiko

AU - Utsunomiya, Tokuichiro

AU - Herndon, David

AU - Pollard, Richard B.

AU - Suzuki, Fujio

PY - 1999/6/15

Y1 - 1999/6/15

N2 - The effect of combination therapy between IL-12 and soluble IL-4R (sIL- 4R) on the established infection of HSV-1 in thermally injured mice (TI mice) was investigated. All of the TI mice infected with lethal amounts of HSV-1 died when IL-12 was given therapeutically at a dose of 500 U/mouse. However, 80% of these mice treated prophylactically with IL-12 survived compared with 0% survival of the same mice treated with saline. The therapeutic administration of IL-12 to TI mice currently infected with HSV-1 caused an 80% survival of these mice when the treatment was combined with sIL-4R. Although IL-12 did not stimulate IFN-γ production in cultures of splenic T cells from TI mice, IFN-γ was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R. After stimulation with anti-CD3 mAb, splenic T cells from TI mice with the established infection of HSV-1 produced IL-4 into their culture fluids. However, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination. The results obtained herein indicate that the efficacies of the combination therapy against the established infection of HSV-1 may result from the IFN- γ production stimulated by IL-12 in TI mice that are treated with sIL-4R for reducing burn-associated type 2 T cell responses.

AB - The effect of combination therapy between IL-12 and soluble IL-4R (sIL- 4R) on the established infection of HSV-1 in thermally injured mice (TI mice) was investigated. All of the TI mice infected with lethal amounts of HSV-1 died when IL-12 was given therapeutically at a dose of 500 U/mouse. However, 80% of these mice treated prophylactically with IL-12 survived compared with 0% survival of the same mice treated with saline. The therapeutic administration of IL-12 to TI mice currently infected with HSV-1 caused an 80% survival of these mice when the treatment was combined with sIL-4R. Although IL-12 did not stimulate IFN-γ production in cultures of splenic T cells from TI mice, IFN-γ was produced by stimulation with IL-12 when the producer cells were prepared from TI mice that had been treated previously with sIL-4R. After stimulation with anti-CD3 mAb, splenic T cells from TI mice with the established infection of HSV-1 produced IL-4 into their culture fluids. However, IL-4 was not produced by splenic T cells that were prepared from the same infected mice treated with IL-12 and sIL-4R in combination. The results obtained herein indicate that the efficacies of the combination therapy against the established infection of HSV-1 may result from the IFN- γ production stimulated by IL-12 in TI mice that are treated with sIL-4R for reducing burn-associated type 2 T cell responses.

UR - http://www.scopus.com/inward/record.url?scp=0033564667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033564667&partnerID=8YFLogxK

M3 - Article

VL - 162

SP - 7148

EP - 7154

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -