Thermoregulated expression of a cloned congo red binding activity gene of Shigella flexneri

Charles E. Chambers, Sharon L. Stockman, David Niesel

Research output: Contribution to journalLetter

Abstract

Abstract A 2.15‐kb Pst I fragment of the 140‐megadalton (MDa) plasmid of Shigella flexneri which encodes Congo red (CR) dye binding activity was isolated by recombinant techniques. The recombinant plasmid was shown to encode the structural gene for CR binding but failed to restore the ability of noninvasive strains to penetrate HeLa cell monolayers. A partial restriction map was constructed and polypeptides specified by the 140‐MDa insert DNA identified in a minicell system. The putative polypeptide responsible for CR binding activity was identified by its thermoregulated expression in minicells. The Mr of the putative CR binding protein was 24000.

Original languageEnglish (US)
Pages (from-to)281-286
Number of pages6
JournalFEMS Microbiology Letters
Volume28
Issue number3
DOIs
StatePublished - 1985

Keywords

  • HeLa cell invasion
  • minicells
  • restriction mapping
  • virulence plasmid

ASJC Scopus subject areas

  • Microbiology
  • Medicine(all)
  • Molecular Biology
  • Genetics

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