Three different neutrophil subsets exhibited in mice with different susceptibilities to infection by methicillin-resistant Staphylococcus aureus

Yasuhiro Tsuda, Hitoshi Takahashi, Makiko Kobayashi, Toshiaki Hanafusa, David Herndon, Fujio Suzuki

Research output: Contribution to journalArticle

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Abstract

Neutrophils (PMN) have been described as critical effector cells in the host's antibacterial innate immunities. However, the classification of murine PMNs remains unclear. Here, we show that in addition to normal PMN (PMN-N), there are at least two distinct subsets of PMNs (PMN-I and PMN-II) distinguished as follows: (1) cytokine and chemokine production (PMN-I, IL-12/CCL3; PMN-II, IL-10/CCL2; PMN-N, no cytokine/chemokine production), (2) macrophage activation (PMN-I, classically activated macrophages; PMN-II, alternatively activated macrophages; PMN-N, no effect on macrophage activation), (3) Toll-like receptor (TLR) expression (PMN-I, TLR2/TLR4/TLR5/TLR8; PMN-II, TLR2/TLR4/TLR7/TLR9; PMN-N, TLR2/TLR4/TLR9), and (4) surface antigen expression (PMN-I, CD49d +CD11b-; PMN-II, CD49d-CD11b+; PMN-N, CD49d-CD11b-). PMN-I was obtained from MRSA (methicillin-resistant Staphylococcus aureus)-resistant hosts, while MRSA-sensitive hosts were a source of PMN-II. PMN-N was obtained from naive mice. Anti-MRSA innate immunities might be influenced differently by these biochemically and physically distinguished PMNs. PMN-N may convert to PMN-I or PMN-II in response to host circumstance.

Original languageEnglish
Pages (from-to)215-226
Number of pages12
JournalImmunity
Volume21
Issue number2
DOIs
StatePublished - Aug 2004

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Methicillin-Resistant Staphylococcus aureus
Neutrophils
Macrophage Activation
Chemokines
Innate Immunity
Infection
Macrophages
Toll-Like Receptor 3
Cytokines
Surface Antigens
Interleukin-12
Interleukin-10

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Three different neutrophil subsets exhibited in mice with different susceptibilities to infection by methicillin-resistant Staphylococcus aureus. / Tsuda, Yasuhiro; Takahashi, Hitoshi; Kobayashi, Makiko; Hanafusa, Toshiaki; Herndon, David; Suzuki, Fujio.

In: Immunity, Vol. 21, No. 2, 08.2004, p. 215-226.

Research output: Contribution to journalArticle

Tsuda, Yasuhiro ; Takahashi, Hitoshi ; Kobayashi, Makiko ; Hanafusa, Toshiaki ; Herndon, David ; Suzuki, Fujio. / Three different neutrophil subsets exhibited in mice with different susceptibilities to infection by methicillin-resistant Staphylococcus aureus. In: Immunity. 2004 ; Vol. 21, No. 2. pp. 215-226.
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AB - Neutrophils (PMN) have been described as critical effector cells in the host's antibacterial innate immunities. However, the classification of murine PMNs remains unclear. Here, we show that in addition to normal PMN (PMN-N), there are at least two distinct subsets of PMNs (PMN-I and PMN-II) distinguished as follows: (1) cytokine and chemokine production (PMN-I, IL-12/CCL3; PMN-II, IL-10/CCL2; PMN-N, no cytokine/chemokine production), (2) macrophage activation (PMN-I, classically activated macrophages; PMN-II, alternatively activated macrophages; PMN-N, no effect on macrophage activation), (3) Toll-like receptor (TLR) expression (PMN-I, TLR2/TLR4/TLR5/TLR8; PMN-II, TLR2/TLR4/TLR7/TLR9; PMN-N, TLR2/TLR4/TLR9), and (4) surface antigen expression (PMN-I, CD49d +CD11b-; PMN-II, CD49d-CD11b+; PMN-N, CD49d-CD11b-). PMN-I was obtained from MRSA (methicillin-resistant Staphylococcus aureus)-resistant hosts, while MRSA-sensitive hosts were a source of PMN-II. PMN-N was obtained from naive mice. Anti-MRSA innate immunities might be influenced differently by these biochemically and physically distinguished PMNs. PMN-N may convert to PMN-I or PMN-II in response to host circumstance.

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