Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

Varatharasa Thiviyanthan, Nishantha Illangasekare, Elliott Gozansky, Frank Zhu, Neocles B. Leontis, Bruce A. Luxon, David G. Gorenstein

    Research output: Contribution to journalArticle

    Abstract

    A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

    Original languageEnglish (US)
    Pages (from-to)167-180
    Number of pages14
    JournalACS Symposium Series
    Volume682
    StatePublished - 1998

    Fingerprint

    Biomolecules
    Computational efficiency
    DNA
    Spectroscopy

    ASJC Scopus subject areas

    • Chemistry(all)

    Cite this

    Thiviyanthan, V., Illangasekare, N., Gozansky, E., Zhu, F., Leontis, N. B., Luxon, B. A., & Gorenstein, D. G. (1998). Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction. ACS Symposium Series, 682, 167-180.

    Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction. / Thiviyanthan, Varatharasa; Illangasekare, Nishantha; Gozansky, Elliott; Zhu, Frank; Leontis, Neocles B.; Luxon, Bruce A.; Gorenstein, David G.

    In: ACS Symposium Series, Vol. 682, 1998, p. 167-180.

    Research output: Contribution to journalArticle

    Thiviyanthan, V, Illangasekare, N, Gozansky, E, Zhu, F, Leontis, NB, Luxon, BA & Gorenstein, DG 1998, 'Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction', ACS Symposium Series, vol. 682, pp. 167-180.
    Thiviyanthan V, Illangasekare N, Gozansky E, Zhu F, Leontis NB, Luxon BA et al. Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction. ACS Symposium Series. 1998;682:167-180.
    Thiviyanthan, Varatharasa ; Illangasekare, Nishantha ; Gozansky, Elliott ; Zhu, Frank ; Leontis, Neocles B. ; Luxon, Bruce A. ; Gorenstein, David G. / Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction. In: ACS Symposium Series. 1998 ; Vol. 682. pp. 167-180.
    @article{5b0fd8ffe0fa4331b3d2cc21c6d8e35e,
    title = "Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction",
    abstract = "A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.",
    author = "Varatharasa Thiviyanthan and Nishantha Illangasekare and Elliott Gozansky and Frank Zhu and Leontis, {Neocles B.} and Luxon, {Bruce A.} and Gorenstein, {David G.}",
    year = "1998",
    language = "English (US)",
    volume = "682",
    pages = "167--180",
    journal = "ACS Symposium Series",
    issn = "0097-6156",
    publisher = "American Chemical Society",

    }

    TY - JOUR

    T1 - Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

    AU - Thiviyanthan, Varatharasa

    AU - Illangasekare, Nishantha

    AU - Gozansky, Elliott

    AU - Zhu, Frank

    AU - Leontis, Neocles B.

    AU - Luxon, Bruce A.

    AU - Gorenstein, David G.

    PY - 1998

    Y1 - 1998

    N2 - A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

    AB - A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

    UR - http://www.scopus.com/inward/record.url?scp=0346615660&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0346615660&partnerID=8YFLogxK

    M3 - Article

    AN - SCOPUS:0346615660

    VL - 682

    SP - 167

    EP - 180

    JO - ACS Symposium Series

    JF - ACS Symposium Series

    SN - 0097-6156

    ER -