Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

Varatharasa Thiviyanthan; Nishantha Illangasekare; Elliott Gozansky; Frank Zhu; Neocles B. Leontis; Bruce A. Luxon; David G. Gorenstein

Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

Varatharasa Thiviyanthan
, Nishantha Illangasekare
, Elliott Gozansky
, Frank Zhu
, Neocles B. Leontis
, Bruce A. Luxon
, David G. Gorenstein

Research output: Contribution to journal › Article › peer-review

Abstract

A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

Original language	English (US)
Pages (from-to)	167-180
Number of pages	14
Journal	ACS Symposium Series
Volume	682
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Chemical Engineering

Cite this

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title = "Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction",

abstract = "A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.",

author = "Varatharasa Thiviyanthan and Nishantha Illangasekare and Elliott Gozansky and Frank Zhu and Leontis, \{Neocles B.\} and Luxon, \{Bruce A.\} and Gorenstein, \{David G.\}",

year = "1998",

language = "English (US)",

volume = "682",

pages = "167--180",

journal = "ACS Symposium Series",

issn = "0097-6156",

publisher = "American Chemical Society",

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T1 - Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

AU - Thiviyanthan, Varatharasa

AU - Illangasekare, Nishantha

AU - Gozansky, Elliott

AU - Zhu, Frank

AU - Leontis, Neocles B.

AU - Luxon, Bruce A.

AU - Gorenstein, David G.

PY - 1998

Y1 - 1998

N2 - A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

AB - A computationally efficient hybrid-hybrid relaxation matrix refinement methodology using 3D NOESY-NOESY data is described. The methodology was tested on simulated data, derived from the Dickerson dodecamer, and used to refine the structure of a DNA three way junction from experimentally determined 3D NOESY-NOESY volume data. Our results indicate that the 3D hybrid-hybrid MORASS methodology, by combining the spectral dispersion of 3D NOESY-NOESY spectroscopy and the computational efficiency of 2D refinement programs, provides an accurate and robust means for structure determination of large biomolecules.

UR - https://www.scopus.com/pages/publications/0346615660

UR - https://www.scopus.com/pages/publications/0346615660#tab=citedBy

M3 - Article

AN - SCOPUS:0346615660

SN - 0097-6156

VL - 682

SP - 167

EP - 180

JO - ACS Symposium Series

JF - ACS Symposium Series

ER -

Three-Dimensional NOESY-NOESY Hybrid-Hybrid Matrix Refinement of a DNA Three-Way Junction

Abstract

ASJC Scopus subject areas

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