Three-dimensional organization of Rift Valley fever virus revealed by cryoelectron tomography

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Abstract

Rift Valley fever virus (RVFV) is a member of the Bunyaviridae virus family (genus Phlebovirus) and is considered to be one of the most important pathogens in Africa, causing viral zoonoses in livestock and humans. Here, we report the characterization of the three-dimensional structural organization of RVFV vaccine strain MP-12 by cryoelectron tomography. Vitrified-hydrated virions were found to be spherical, with an average diameter of 100 nm. The virus glycoproteins formed cylindrical hollow spikes that clustered into distinct capsomeres. In contrast to previous assertions that RVFV is pleomorphic, the structure of RVFV MP-12 was found to be highly ordered. The three-dimensional map was resolved to a resolution of 6.1 nm, and capsomeres were observed to be arranged on the virus surface in an icosahedral lattice with clear T=12 quasisymmetry. All icosahedral symmetry axes were visible in self-rotation functions calculated using the Fourier transform of the RVFV MP-12 tomogram. To the best of our knowledge, a triangulation number of 12 had previously been reported only for Uukuniemi virus, a bunyavirus also within the Phlebovirus genus. The results presented in this study demonstrate that RVFV MP-12 possesses T=12 icosahedral symmetry and suggest that other members of the Phlebovirus genus, as well as of the Bunyaviridae family, may adopt icosahedral symmetry. Knowledge of the virus architecture may provide a structural template to develop vaccines and diagnostics, since no effective anti-RVFV treatments are available for human use.

Original languageEnglish (US)
Pages (from-to)10341-10348
Number of pages8
JournalJournal of Virology
Volume82
Issue number21
DOIs
StatePublished - Nov 2008

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Rift Valley fever virus
tomography
Tomography
Phlebovirus
Bunyaviridae
Viruses
viruses
Uukuniemi virus
Vaccines
Orthobunyavirus
vaccines
Zoonoses
Livestock
Fourier Analysis
zoonoses
virion
Virion
glycoproteins
Glycoproteins
livestock

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Three-dimensional organization of Rift Valley fever virus revealed by cryoelectron tomography. / Freiberg, Alexander; Sherman, Michael; Morais, Marc; Holbrook, Michael R.; Watowich, Stanley.

In: Journal of Virology, Vol. 82, No. 21, 11.2008, p. 10341-10348.

Research output: Contribution to journalArticle

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