Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host

Petr Leiman, Paul R. Chipman, Victor A. Kostyuchenko, Vadim V. Mesyanzhinov, Michael G. Rossmann

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

The contractile tail of bacteriophage T4 undergoes major structural transitions when the virus attaches to the host cell surface. The baseplate at the distal end of the tail changes from a hexagonal to a star shape. This causes the sheath around the tail tube to contract and the tail tube to protrude from the baseplate and pierce the outer cell membrane and the cell wall before reaching the inner cell membrane for subsequent viral DNA injection. Analogously, the T4 tail can be contracted by treatment with 3 M urea. The structure of the T4 contracted tail, including the head-tail joining region, has been determined by cryo-electron microscopy to 17 Å resolution. This 1200 Å-long, 20 MDa structure has been interpreted in terms of multiple copies of its approximately 20 component proteins. A comparison with the metastable hexagonal baseplate of the mature virus shows that the baseplate proteins move as rigid bodies relative to each other during the structural change.

Original languageEnglish (US)
Pages (from-to)419-429
Number of pages11
JournalCell
Volume118
Issue number4
DOIs
StatePublished - Aug 20 2004
Externally publishedYes

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Bacteriophage T4
Bacteriophages
Cell membranes
Viruses
Cell Membrane
Cryoelectron Microscopy
Viral DNA
Infection
Joining
Cell Wall
Electron microscopy
Stars
Urea
Proteins
Head
Cells
Injections
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Leiman, P., Chipman, P. R., Kostyuchenko, V. A., Mesyanzhinov, V. V., & Rossmann, M. G. (2004). Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. Cell, 118(4), 419-429. https://doi.org/10.1016/j.cell.2004.07.022

Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. / Leiman, Petr; Chipman, Paul R.; Kostyuchenko, Victor A.; Mesyanzhinov, Vadim V.; Rossmann, Michael G.

In: Cell, Vol. 118, No. 4, 20.08.2004, p. 419-429.

Research output: Contribution to journalArticle

Leiman, P, Chipman, PR, Kostyuchenko, VA, Mesyanzhinov, VV & Rossmann, MG 2004, 'Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host', Cell, vol. 118, no. 4, pp. 419-429. https://doi.org/10.1016/j.cell.2004.07.022
Leiman, Petr ; Chipman, Paul R. ; Kostyuchenko, Victor A. ; Mesyanzhinov, Vadim V. ; Rossmann, Michael G. / Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host. In: Cell. 2004 ; Vol. 118, No. 4. pp. 419-429.
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