Three-dimensional solution structure of a single zinc finger DNA-binding domain

Min S. Lee, Garry P. Gippert, Kizhake V. Soman, David A. Case, Peter E. Wright

Research output: Contribution to journalArticle

508 Citations (Scopus)

Abstract

The three-dimensional solution structure of a zinc finger nucleic acid binding motif has been determined by nuclear magnetic resonance (NMR) spectroscopy. Spectra of a synthetic peptide corresponding to a single zinc finger from the Xenopus protein Xfin yielded distance and dihedral angle constraints that were used to generate structures from distance geometry and restrained molecular dynamics calculations. The zinc finger is an independently folded domain with a compact globular structure in which the zinc atom is bound by two cysteine and two histidine ligands. The polypeptide backbone fold consists of a well-defined helix, starting as α and ending as 3 10 helix, packed against two β strands that are arranged in a hairpin structure. A high density of basic and polar amino acid side chains on the exposed face of the helix are probably involved in DNA binding.

Original languageEnglish (US)
Pages (from-to)635-637
Number of pages3
JournalScience
Volume245
Issue number4918
StatePublished - 1989
Externally publishedYes

Fingerprint

Zinc Fingers
DNA
Xenopus Proteins
Basic Amino Acids
Peptides
Molecular Dynamics Simulation
Histidine
Nucleic Acids
Cysteine
Zinc
Magnetic Resonance Spectroscopy
Ligands

ASJC Scopus subject areas

  • General

Cite this

Lee, M. S., Gippert, G. P., Soman, K. V., Case, D. A., & Wright, P. E. (1989). Three-dimensional solution structure of a single zinc finger DNA-binding domain. Science, 245(4918), 635-637.

Three-dimensional solution structure of a single zinc finger DNA-binding domain. / Lee, Min S.; Gippert, Garry P.; Soman, Kizhake V.; Case, David A.; Wright, Peter E.

In: Science, Vol. 245, No. 4918, 1989, p. 635-637.

Research output: Contribution to journalArticle

Lee, MS, Gippert, GP, Soman, KV, Case, DA & Wright, PE 1989, 'Three-dimensional solution structure of a single zinc finger DNA-binding domain', Science, vol. 245, no. 4918, pp. 635-637.
Lee MS, Gippert GP, Soman KV, Case DA, Wright PE. Three-dimensional solution structure of a single zinc finger DNA-binding domain. Science. 1989;245(4918):635-637.
Lee, Min S. ; Gippert, Garry P. ; Soman, Kizhake V. ; Case, David A. ; Wright, Peter E. / Three-dimensional solution structure of a single zinc finger DNA-binding domain. In: Science. 1989 ; Vol. 245, No. 4918. pp. 635-637.
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