Three-dimensional structure of bacteriophage T4 baseplate

Victor A. Kostyuchenko, Petr Leiman, Paul R. Chipman, Shuji Kanamaru, Mark J. Van Raaij, Fumio Arisaka, Vadim V. Mesyanzhinov, Michael G. Rossmann

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 Å by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure ∼520 Å in diameter and ∼270 Å long, assembled around a central hub. A 940 Å-long and 96 Å-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.

Original languageEnglish (US)
Pages (from-to)688-693
Number of pages6
JournalNature Structural Biology
Volume10
Issue number9
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

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Bacteriophage T4
Bacteriophages
Domes
Cryoelectron Microscopy
Piercing
Viral DNA
Needles
Microscopic examination
Proteins
Equipment and Supplies
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Kostyuchenko, V. A., Leiman, P., Chipman, P. R., Kanamaru, S., Van Raaij, M. J., Arisaka, F., ... Rossmann, M. G. (2003). Three-dimensional structure of bacteriophage T4 baseplate. Nature Structural Biology, 10(9), 688-693. https://doi.org/10.1038/nsb970

Three-dimensional structure of bacteriophage T4 baseplate. / Kostyuchenko, Victor A.; Leiman, Petr; Chipman, Paul R.; Kanamaru, Shuji; Van Raaij, Mark J.; Arisaka, Fumio; Mesyanzhinov, Vadim V.; Rossmann, Michael G.

In: Nature Structural Biology, Vol. 10, No. 9, 01.09.2003, p. 688-693.

Research output: Contribution to journalArticle

Kostyuchenko, VA, Leiman, P, Chipman, PR, Kanamaru, S, Van Raaij, MJ, Arisaka, F, Mesyanzhinov, VV & Rossmann, MG 2003, 'Three-dimensional structure of bacteriophage T4 baseplate', Nature Structural Biology, vol. 10, no. 9, pp. 688-693. https://doi.org/10.1038/nsb970
Kostyuchenko VA, Leiman P, Chipman PR, Kanamaru S, Van Raaij MJ, Arisaka F et al. Three-dimensional structure of bacteriophage T4 baseplate. Nature Structural Biology. 2003 Sep 1;10(9):688-693. https://doi.org/10.1038/nsb970
Kostyuchenko, Victor A. ; Leiman, Petr ; Chipman, Paul R. ; Kanamaru, Shuji ; Van Raaij, Mark J. ; Arisaka, Fumio ; Mesyanzhinov, Vadim V. ; Rossmann, Michael G. / Three-dimensional structure of bacteriophage T4 baseplate. In: Nature Structural Biology. 2003 ; Vol. 10, No. 9. pp. 688-693.
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