Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina

Michael Sherman, E. V. Orlova, E. A. Smirnova, S. Hovmoller, N. A. Zorin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped complex containing six each of the large (62-kDa) and small (26-kDa) subunits. The complex is very open, with six well-separated dumbbell-shaped masses surrounding a large cylindrical hole. Each dumbbell is interpreted as consisting of one large and one small subunit.

Original languageEnglish (US)
Pages (from-to)2576-2580
Number of pages5
JournalJournal of Bacteriology
Volume173
Issue number8
StatePublished - 1991
Externally publishedYes

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Thiocapsa roseopersicina
Hydrogenase
Nickel
Enzymes
Electron Microscopy

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Sherman, M., Orlova, E. V., Smirnova, E. A., Hovmoller, S., & Zorin, N. A. (1991). Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina. Journal of Bacteriology, 173(8), 2576-2580.

Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina. / Sherman, Michael; Orlova, E. V.; Smirnova, E. A.; Hovmoller, S.; Zorin, N. A.

In: Journal of Bacteriology, Vol. 173, No. 8, 1991, p. 2576-2580.

Research output: Contribution to journalArticle

Sherman, M, Orlova, EV, Smirnova, EA, Hovmoller, S & Zorin, NA 1991, 'Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina', Journal of Bacteriology, vol. 173, no. 8, pp. 2576-2580.
Sherman, Michael ; Orlova, E. V. ; Smirnova, E. A. ; Hovmoller, S. ; Zorin, N. A. / Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina. In: Journal of Bacteriology. 1991 ; Vol. 173, No. 8. pp. 2576-2580.
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