Thrombin-induced gap formation in confluent endothelial cell monolayers in vitro

Michael Laposata, D. K. Dovnarsky, H. Shin

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

When thrombin is incubated with confluent monolayers of human umbilical vein endothelial cells in vitro, there is a change in the shape of the endothelial cells that results in gaps in the monolayer disrupting the integrity of the endothelium and exposing the subendothelium. Using a grid assay to measure this phenomenon, we observed that up to 80% of the surface area once covered by cells was uncovered after a 15-min incubation with 10-2 U/ml (10-10 M) thrombin. The effect was apparent within 2 min and did not remove cells from the surface of the culture dish. The gaps in the monolayer completely disappeared within 2 hr after exposure to thrombin. The effect of thrombin was inhibited by preincubation of thrombin with hirudin or antithrombin III plush heparin or by preincubation of the monolayers with dibutyryl cyclic adenosine monophosphate (dbcAMP). Histamine also induced gap formation in endothelial cell monolayers. Both pyrilamine and cimetidine had no effect on thrombin-induced gap formation. Intact monolayers were not disrupted by bradykinin, serotonin, C5a, or C3a. Our results suggest that small amounts of thrombin can induce repeated and transient exposure of the subendothelium, a situation believed to be conducive to atherogenesis and thrombosis.

Original languageEnglish (US)
Pages (from-to)549-556
Number of pages8
JournalBlood
Volume62
Issue number3
StatePublished - 1983
Externally publishedYes

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Endothelial cells
Thrombin
Monolayers
Endothelial Cells
Pyrilamine
Hirudins
Antithrombin III
Cimetidine
Human Umbilical Vein Endothelial Cells
Bradykinin
In Vitro Techniques
Cyclic AMP
Histamine
Endothelium
Heparin
Assays
Serotonin
Atherosclerosis
Thrombosis
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

Thrombin-induced gap formation in confluent endothelial cell monolayers in vitro. / Laposata, Michael; Dovnarsky, D. K.; Shin, H.

In: Blood, Vol. 62, No. 3, 1983, p. 549-556.

Research output: Contribution to journalArticle

Laposata, M, Dovnarsky, DK & Shin, H 1983, 'Thrombin-induced gap formation in confluent endothelial cell monolayers in vitro', Blood, vol. 62, no. 3, pp. 549-556.
Laposata, Michael ; Dovnarsky, D. K. ; Shin, H. / Thrombin-induced gap formation in confluent endothelial cell monolayers in vitro. In: Blood. 1983 ; Vol. 62, No. 3. pp. 549-556.
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