Time course of leukocyte adhesion to endothelium in ischemia-reperfusion

Vincent J. Milazzo, Robert J. Ferrante, Frederick Sabido, Michael Silva, Robert W. Hobson, Walter N. Duran

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Adhesion of leukocytes (L) to microvascular endothelium (E) is a required step in the L-E interaction leading to tissue injury in ischemia-reperfusion. To assess the optimum period for therapy aimed at ameliorating negative effects of this required step, we investigated the time course of L-E adhesion in the hamster cheek pouch using 2 hr of ischemia and 1 hr of reperfusion in our model of I-R injury (Am. J. Physiol. 261: 1626, 1991). Leukocytes adhering (stationary for ≥30 sec) to postcapillary venules (15- 30 μm in diameter) were counted after labeling with acridine orange. Prior to the induction of ischemia, there were no significant differences in the number of adherent leukocytes in each area chosen for study (1.9 ± 0.6 vs 2.0 ± 0.3; mean number of leukocytes/100-μm vessel length ± SD). After 10 and 20 min of reperfusion there was no significant difference in leukocyte adhesion in the ischemic area relative to the control (2.7 ± 0.5 vs 2.8 ± 0.8, and 5.3 ± 2.8 vs 2.4 ± 0.6, respectively). Leukocyte adherence increased significantly after 30 min of reperfusion and remained elevated at 1 hr of reperfusion in the postischemic area relative to the nonischemic control area (7.8 ± 1.3 vs 3.6 ± 0.6, and 8.3 ± 0.8 vs 4.1 ± 0.6, respectively; P < 0.01). Leukocyte adhesion in the postischemic area after 30 min reperfusion was not significantly different from the adhesion at the end of 1 hr reperfusion. These data suggest that (1) peak leukocyte adhesion occurs after 30 min of normal reperfusion and (2) postischemic therapeutic intervention may be most beneficial when instituted within this early time period.

Original languageEnglish (US)
Pages (from-to)139-142
Number of pages4
JournalJournal of Surgical Research
Volume61
Issue number1
DOIs
StatePublished - Feb 15 1996
Externally publishedYes

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Reperfusion
Endothelium
Leukocytes
Ischemia
Leukocyte Count
Acridine Orange
Venules
Cheek
Reperfusion Injury
Cricetinae
Wounds and Injuries
Therapeutics

ASJC Scopus subject areas

  • Surgery

Cite this

Milazzo, V. J., Ferrante, R. J., Sabido, F., Silva, M., Hobson, R. W., & Duran, W. N. (1996). Time course of leukocyte adhesion to endothelium in ischemia-reperfusion. Journal of Surgical Research, 61(1), 139-142. https://doi.org/10.1006/jsre.1996.0094

Time course of leukocyte adhesion to endothelium in ischemia-reperfusion. / Milazzo, Vincent J.; Ferrante, Robert J.; Sabido, Frederick; Silva, Michael; Hobson, Robert W.; Duran, Walter N.

In: Journal of Surgical Research, Vol. 61, No. 1, 15.02.1996, p. 139-142.

Research output: Contribution to journalArticle

Milazzo, VJ, Ferrante, RJ, Sabido, F, Silva, M, Hobson, RW & Duran, WN 1996, 'Time course of leukocyte adhesion to endothelium in ischemia-reperfusion', Journal of Surgical Research, vol. 61, no. 1, pp. 139-142. https://doi.org/10.1006/jsre.1996.0094
Milazzo, Vincent J. ; Ferrante, Robert J. ; Sabido, Frederick ; Silva, Michael ; Hobson, Robert W. ; Duran, Walter N. / Time course of leukocyte adhesion to endothelium in ischemia-reperfusion. In: Journal of Surgical Research. 1996 ; Vol. 61, No. 1. pp. 139-142.
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