Time course of skeletal muscle glucose uptake during euglycemic hyperinsulinemia in the anesthetized rabbit: A fluorine-18-2-deoxy-2-fluoro- D-glucose study

K. A. Mossberg, H. Taegtmeyer

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    Abstract

    Since skeletal muscle has been implicated as the major site of insulin resistance, the purpose of this study was to examine in detail the time course of muscle glucose uptake during the onset and maintenance of euglycemic hyperinsulinemia. Uptake of 18F-2-deoxy-2-fluoro-D-glucose (FDG) by the thigh muscle of an anesthetized rabbit was monitored by a single pair of coincidence photon detectors. Graphical analysis of tissue and plasma radioactivity concentrations was performed to derive fractional rates of FDG phosphorylation continuously. FDG phosphorylation rates were determined during rest (glucose 7 mM, insulin 5-10 μU/ml) and subsequent 5-min intervals under conditions of euglycemic hyperinsulinemia (glucose 6-8 mM; insulin 350-400 μU/ml plasma). FDG phosphorylation did not increase above resting control levels until 5.5 ± 1.5 min after intravenous insulin administration. After 20-30 min of hyperinsulinemia, FDG phosphorylation and calculated glucose metabolic rates were increased by 50%. At 35-40 min of the clamp in place, there was a second increase in tracer phosphorylation which plateaued at 200% of control (p < 0.01) and remained at this level for the remainder of the experiment. In conclusion, we have described a method for making rapid, serial estimates of insulin-mediated skeletal muscle glucose uptake. We suggest that appraisal of the time course of glucose uptake with FDG will aid in the understanding of normal and pathophysiologic states of insulin action in vivo.

    Original languageEnglish (US)
    Pages (from-to)1523-1529
    Number of pages7
    JournalJournal of Nuclear Medicine
    Volume33
    Issue number8
    StatePublished - 1992

    Fingerprint

    Fluorine
    Fluorodeoxyglucose F18
    Hyperinsulinism
    Skeletal Muscle
    Rabbits
    Glucose
    Phosphorylation
    Insulin
    Muscles
    Thigh
    Photons
    Intravenous Administration
    Radioactivity
    Insulin Resistance
    Maintenance

    ASJC Scopus subject areas

    • Radiological and Ultrasound Technology

    Cite this

    Time course of skeletal muscle glucose uptake during euglycemic hyperinsulinemia in the anesthetized rabbit : A fluorine-18-2-deoxy-2-fluoro- D-glucose study. / Mossberg, K. A.; Taegtmeyer, H.

    In: Journal of Nuclear Medicine, Vol. 33, No. 8, 1992, p. 1523-1529.

    Research output: Contribution to journalArticle

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