Time-dependent and organ-specific changes in mitochondrial function, mitochondrial DNA integrity, oxidative stress and mononuclear cell infiltration in a mouse model of burn injury

Bartosz Szczesny, Attila Brunyánszki, Akbar Ahmad, Gabor Oláh, Craig Porter, Tracy Toliver-Kinsky, Labros Sidossis, David Herndon, Csaba Szabo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Severe thermal injury induces a pathophysiological response that affects most of the organs within the body; liver, heart, lung, skeletal muscle among others, with inflammation and hyper-metabolism as a hallmark of the post-burn damage. Oxidative stress has been implicated as a key component in development of inflammatory and metabolic responses induced by burn. The goal of the current study was to evaluate several critical mitochondrial functions in a mouse model of severe burn injury. Mitochondrial bioenergetics, measured by Extracellular Flux Analyzer, showed a time dependent, post-burn decrease in basal respiration and ATP-turnover but enhanced maximal respiratory capacity in mitochondria isolated from the liver and lung of animals subjected to burn injury. Moreover, we detected a tissue-specific degree of DNA damage, particularly of the mitochondrial DNA, with the most profound effect detected in lungs and hearts of mice subjected to burn injury. Increased mitochondrial biogenesis in lung tissue in response to burn injury was also observed. Burn injury also induced time dependent increases in oxidative stress (measured by amount of malondialdehyde) and neutrophil infiltration (measured by myeloperoxidase activity), particularly in lung and heart. Tissue mononuclear cell infiltration was also confirmed by immunohistochemistry. The amount of poly(ADP-ribose) polymers decreased in the liver, but increased in the heart in later time points after burn. All of these biochemical changes were also associated with histological alterations in all three organs studied. Finally, we detected a significant increase in mitochondrial DNA fragments circulating in the blood immediately post-burn. There was no evidence of systemic bacteremia, or the presence of bacterial DNA fragments at any time after burn injury. The majority of the measured parameters demonstrated a sustained elevation even at 20-40 days post injury suggesting a long-lasting effect of thermal injury on organ function. The current data show that there are marked timedependent and tissue-specific alterations in mitochondrial function induced by thermal injury, and suggest that mitochondria-specific damage is one of the earliest responses to burn injury. Mitochondria may be potential therapeutic targets in the future experimental therapy of burns.

Original languageEnglish (US)
Article numbere0143730
JournalPLoS One
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2015

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burns (injuries)
Oxidative stress
Mitochondrial DNA
Infiltration
Mitochondria
Oxidative Stress
mitochondrial DNA
oxidative stress
animal models
Liver
Tissue
heat injury
Wounds and Injuries
lungs
heart
Burns
cells
mitochondria
Poly Adenosine Diphosphate Ribose
Bacterial DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Time-dependent and organ-specific changes in mitochondrial function, mitochondrial DNA integrity, oxidative stress and mononuclear cell infiltration in a mouse model of burn injury. / Szczesny, Bartosz; Brunyánszki, Attila; Ahmad, Akbar; Oláh, Gabor; Porter, Craig; Toliver-Kinsky, Tracy; Sidossis, Labros; Herndon, David; Szabo, Csaba.

In: PLoS One, Vol. 10, No. 12, e0143730, 01.12.2015.

Research output: Contribution to journalArticle

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