Tissue-engineered intrasynovial tendons: Optimization of acellularization and seeding

Andrew Y. Zhang, Steven J. Bates, Ellen Morrow, Hung Pham, Bryant Pham, James Chang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The purpose of this research was to develop a tissue-engineered intrasynovial flexor tendon construct with the use an acellularized flexor tendon scaffold repopulated with intrasynovial tendon cells. New Zealand white rabbit intrasynovial flexor tendons were acellularized by the following methods: high concentration NaCl + SDS, Trypsin/EDTA, Trypsin/EDTA Triton X-100, Triton X-100, Triton X-100 + SDS, and freezing -70 °C followed by Trypsin/EDTA + Triton X-100. Epitenon and endotenon cells were also isolated from rabbit intrasynovial tendons and expanded in culture. Acellularized tendon scaffolds were then reseeded with these cells. A subset of epitenon and endotenon cells was labeled with green and red fluorescent markers, respectively, to further characterize the preferred location of their attachment. Optimal acellularization was achieved by freezing at -70 °C followed by Trypsin/EDTA + Triton X-100. After reseeding, light microscopy of tendon constructs showed attachment of both epitenon and endotenon to the tendon scaffolds, with endotenon cells more likely to be found the core of the scaffold. An intrasynovial tendon construct was developed with the use of acellularized intrasynovial tendons repopulated with intrasynovial tenocytes. These constructs grossly resemble normal intrasynovial tendons, and cells were found both on the surface and the core of the construct histologically. This new construct represents an important first step in developing a viable tissue-engineered flexor tendon.

Original languageEnglish (US)
Pages (from-to)489-498
Number of pages10
JournalJournal of Rehabilitation Research and Development
Volume46
Issue number4
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Tendons
Octoxynol
Edetic Acid
Trypsin
Freezing
Rabbits
Microscopy

Keywords

  • Acellularization
  • Endotenon
  • Epitenon
  • Flexor tendon
  • Flexor tendon sheath
  • Hand surgery
  • Intrasynovial tendon
  • Rabbit model
  • Rehabilitation
  • Reseeding
  • Tissue engineering

ASJC Scopus subject areas

  • Rehabilitation

Cite this

Tissue-engineered intrasynovial tendons : Optimization of acellularization and seeding. / Zhang, Andrew Y.; Bates, Steven J.; Morrow, Ellen; Pham, Hung; Pham, Bryant; Chang, James.

In: Journal of Rehabilitation Research and Development, Vol. 46, No. 4, 2009, p. 489-498.

Research output: Contribution to journalArticle

Zhang, Andrew Y. ; Bates, Steven J. ; Morrow, Ellen ; Pham, Hung ; Pham, Bryant ; Chang, James. / Tissue-engineered intrasynovial tendons : Optimization of acellularization and seeding. In: Journal of Rehabilitation Research and Development. 2009 ; Vol. 46, No. 4. pp. 489-498.
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